| 热效应在淋巴瘤诊断与研究中的应用 |
| |
| 引用本文： | 王晋芬,王全红,焦士兰.热效应在淋巴瘤诊断与研究中的应用[J].肿瘤研究与临床,1995,7(4):228-229. |
| |
| 作者姓名： | 王晋芬 王全红 焦士兰 |
| |
| 作者单位： | 山西省肿瘤医院 |
| |
| 摘 要： | 石蜡包埋组织免痘组化染色是淋巴瘤病理诊断与研究的重要手段之一，由于常规组织处理过程造成抗原封闭与结构改变，使一些抗体不能用于石蜡切片或厦应微弱，我们应用热介导抗原恢复效应引起蛋白结构改变原理，对组织切片在高压铸内进行热处理，使原来只用于冰冻切片的某些抗体现可用于石蜡切片，扩大了抗体的使用范围，提高了免覆组化在淋巴瘤诊断与研究中的应用价值，增强了免疫染色的敏感性，使抗体稀释度增加0～20倍．使多数淋巴瘤得到准确诊断。
|
| 关 键 词： | 诊断 淋巴瘤 抗体 石蜡切片 抗原 冰冻切片 组化染色 蛋白结构 免疫染色 稀释度 |
THE APPLICATION OF THERMAL EFFECT IN DIAGNOSES AND RESEARCHES OF
LYMPHOMAS |
| |
| Wang Jinfen,et al Shanxi Provincial Tumour
Hospital.THE APPLICATION OF THERMAL EFFECT IN DIAGNOSES AND RESEARCHES OF
LYMPHOMAS[J].Cancer Research and Clinic,1995,7(4):228-229. |
| |
| Authors: | Wang Jinfen Shanxi Provincial Tumour
Hospital |
| |
| Abstract: | Unmasking of antigen in paraffin sections has always
been a problem due to rigours ofroutine formalin fixation and
paraffin embedment.With using of microwave oven andpressure
cooker,this problem has been improved.Tissue sections are boiled for
2 minutedunder pressure and stained as usual.Some antibodies that
stained well in frozen sections alsostained well in paraffin sections
after heating pretreatment. The diagnostic application inlymphomas
has been improved by the application of heating pretreatment. |
| |
| Keywords: | thermal effect immunohistochemistry lymphoma |
| 本文献已被 CNKI 维普 等数据库收录！ |
