|
|||||
|
|
| 人胃癌组织RNA的抽提及其C—H—ras原癌基因的表达 | |
| 引用本文： | 章有章 王瑞年. 人胃癌组织RNA的抽提及其C—H—ras原癌基因的表达[J]. 上海交通大学学报(医学版), 1990, 10(4): 304-308 |
| 作者姓名： | 章有章 王瑞年 |
| 作者单位： | 上海第二医科大学生物化学教研室(章有章，钱关祥，钱晓岚，王璐，于丽莉，陈诗书)，上海第二医科大学病理学教研室(王瑞年) |
| 摘 要： | 用自制平板式不锈钢组织粉碎器。直接从手术切除的新鲜人原发性胃癌组织中用异硫氰酸胍-氯化铯超离心法抽提组织总RNA,再用Oligo(dT)纤维素柱分离得到mRNA,以经典的Northern印迹及分子杂交、放射自显影法观察到人原发性胃癌组织中c-H-ras原癌基因的表达强于或等于自身对照的相应正常胃粘膜组织。 |
| 关 键 词： | 胃癌 RNA 原癌基因 |
Extract of RNA from Human Gastric Cancer Tissue and the Expression of the Protooncogene c-H-ras |
|
| Zhang Youzhang,et al. Extract of RNA from Human Gastric Cancer Tissue and the Expression of the Protooncogene c-H-ras[J]. Journal of Shanghai Jiaotong University:Medical Science, 1990, 10(4): 304-308 | |
| Authors: | Zhang Youzhang et al |
| Affiliation: | Zhang Youzhang,et al Department of Biochemistry,Shanghai Second Medical University. Shanghai |
| Abstract: | This article describes a method to extract total RNA, mRNA and an investigation of the expression of c-Ha-ras protooncogene in human primary gastric cancer. The malignant and the corresponding normal gastric mucosal tissue were obtained from gastrectomy patients, broken into small pieces by a plate form stainless stell splitter, pulverized to tissue powder in a mortor with liquid nitrogen, and the tissue power homogenized in guanindium thiocynate with a polytron. Total RNAs was isolated by guanidium-cesium chloride ultracentrifugal method, mRNAs was then isolated from total RNAs by Oligo(dT)cellulose colum chromatography. Castric total RNAs and mRNAs were analysed by Northern blot method. We found that the expression of c-Ha-ras protooncogene in human gastric cancers was higher than or equal to the corresponding normal gastric mucosal tissues and there was no close relationship between the expression of protooncogene and histology of malignant tissue. |
| Keywords: | c-H-ras RNA human primary gastric cancer protooncogene expresssion |
| 本文献已被 CNKI 维普 等数据库收录！ | |