降钙素基因相关肽阳性神经纤维在鼠颞下颌关节中的分布

目的　观察鼠颞下颌关节中降钙素基因相关肽阳性神经纤维的分布特征。方法　将鼠颞下颌关节取出 ,脱钙脱水后恒冷切片 ,以ABC法漂染后进行镜检及半定量分析。结果　除关节盘中心区外 ,颞下颌关节各部位的软组织均有丰富的降钙素基因相关肽阳性纤维 ,主要沿血管尤其是动脉周围分布 ,密度为 :前部盘周附着和关节囊 (45 4 6± 72 8)mm2 、后部 (30 2 7± 43 4)mm2 、外侧 (2 40 2± 2 3 6 )mm2 、内侧 (2 2 9 6± 2 5 0 )mm2 、盘外周区 (2 0 2 4± 35 2 )mm2 。结论　降钙素基因相关肽阳性纤维广泛分布于颞下颌关节中 ,是感觉神经的一部分 ,其密度以关节前部盘周附着及关节囊中最高

Distribution of calcitonin gene-related peptide-like immunoreactive fibres in the temporomandibular joint of rat

OBJECTIVE: To observe the distribution of calcitonin gene-related peptide-like immunoreactive (CGRP-LI) nerve fibres in rat's temporomandibular joint (TMJ). METHODS: The avidin-biotin-peroxidase complex method and image analysis were employed to detect CGRP-LI nerve fibres in frozen sections of TMJs in eight rats. RESULTS: CGRP-LI nerve fibres were plentifully distributed in TMJs except the central disc band and bone, mainly in the periphery of blood vessels, especially arteries. Density: anterior disc attachment and capsule, (454.6 +/- 72.8) mm(2); posterior portion, (302.7 +/- 43.4) mm(2); lateral portion, (240.2 +/- 23.6) mm(2); medial portion, (229.6 +/- 25.0)mm(2); and peripheral portion of disc, (202.4 +/- 35.2) mm(2). CONCLUSION: CGRP-LI nerve fibres were widely distributed in rat TMJ and were a part of trigeminal sensory nerve. Density was highest in anterior disc attachment and capsule.