| 均匀设计优选干蟾中吲哚类生物碱的水提工艺 |
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| 引用本文： | 李伟,王元清,严建业,罗堃. 均匀设计优选干蟾中吲哚类生物碱的水提工艺[J]. 中国中医药现代远程教育, 2014, 0(4): 144-146 |
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| 作者姓名： | 李伟 王元清 严建业 罗堃 |
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| 作者单位： | [1]湖南省湘潭市中心医院,湘潭411100 [2]湖南中医药大学药学院,中药现代化教育厅重点实验室,长沙,410208;中南林业科技大学生命科学与技术学院,生物技术与工程实验室,长沙,410004 [3]湖南中医药大学药学院,中药现代化教育厅重点实验室长沙410208 |
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| 基金项目： | 湖南中医药大学青年基金项目[No：99820001];湖南省高校科技创新团队资助项目[No：湘教通（2010）212号] |
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| 摘 要： | 目的 优选干蟾中吲哚类生物碱的最佳提取工艺.方法 以吲哚类生物碱浸出量、浸膏得率为评价指标,对提取次数进行考察;选择浸泡时间、提取时间、溶媒用量等为影响因素,应用均匀设计优选其最佳提取工艺.结果 最佳工艺条件为：水提3次,浸泡15min,加水量为14倍、12倍、12倍,提取时间为100min、80min、80min.结论 该工艺稳定,可行,能为其工业化生产提供实验依据.
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| 关 键 词： | 干蟾 均匀设计 提取工艺 吲哚类生物碱 |
Optimizing the water extracting conditions of Indole Alkaloids from Siccus Bufo by uniform design |
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| Li Wei,Wang Yuanqing,Yan Jianye,Luo. Optimizing the water extracting conditions of Indole Alkaloids from Siccus Bufo by uniform design[J]. Chinese Medicine Modern Distance Education of China, 2014, 0(4): 144-146 |
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| Authors: | Li Wei Wang Yuanqing Yan Jianye Luo |
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| Affiliation: | Kun( 1.Xiangtan Central Hospital, Hunan Xiangtan provicine,411100,China; 2.Key Lab of Modernization of Chinese Medicine,collega of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,China;Lab of Biotechnology and Engineering,College of Life Science and Technology,Central South University of Forestry and Technology,Changsha 410004,China;) |
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| Abstract: | Objective To study the optimum extracting process for indole alkaloids from Siccus Bufo.Methods The extraction rate of extractum and extraction amount of indole alkaloids of Siccus Bufo were choosen as the assessment index,with solvent volume,macerating time and extracting time as examming factor,the optimum extraction progress was selected with the uniform design.Results The optimum extraction progress conditions were as follows：macerated for 15 min in water,decocting for 3 times,with water of 14,12,12 times of the drug mixture respectively,105 min,80min,80min respectively.Conclusion The extraction process of optimization can extract more indole alkaloids,and can provide the test foundation for production. |
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| Keywords: | Siccus Bufo uniform design extraction process indole alkaloids |
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