| 丹参对高血压病血瘀证血清诱导内皮功能障碍的影响 |
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| 引用本文： | 江小梨,邓波,刘彬,张双伟,张竞之.丹参对高血压病血瘀证血清诱导内皮功能障碍的影响[J].中华中医药学刊,2019(3):638-642. |
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| 作者姓名： | 江小梨 邓波 刘彬 张双伟 张竞之 |
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| 作者单位： | 1.广州医科大学附属第二医院中医科;2.广州市心血管疾病研究所 |
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| 基金项目： | 国家自然科学基金项目(81874418;81774100;81373520;81503523);广东省科技计划项目(2014A020221059;2015A020211040;2017A020215115;2016A020215167) |
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| 摘 要： | 目的:探索丹参对高血压病血瘀证患者血清诱导的内皮功能障碍及腺苷酸活化蛋白激酶(AMPK)活性的影响,并进一步探讨丹参保护内皮功能障碍的潜在作用机制。方法:以人脐静脉内皮细胞(HUVEC)为研究对象,利用高血压病血瘀证患者血清诱导内皮功能障碍,用不同浓度的丹参(50、100μg/mL)处理24 h后,采用试剂盒检测一氧化氮(NO)及内皮素(ET-1)的含量。采用Western Blot检测磷酸化及总的一氧化氮合酶(eNOS)、细胞间黏附分子-1 (ICAM-1)、血管细胞黏附分子-1(VCAM-1)、磷酸化及总的AMPK的表达。采用荧光定量PCR技术检测肿瘤坏死因子(TNF-α)、白细胞介素-1β(IL-1β)的表达。结果:丹参能剂量依赖性的增加NO的含量、降低ET-1的含量、增加eNOS的磷酸化水平,降低内皮细胞黏附分子ICAM-1、VCAM-1的表达,减少单核细胞黏附率,降低炎性细胞因子TNF-α、IL-1β水平,升高AMPK磷酸化水平,与模型组比较差异均具有统计学意义(P<0.05)。结论:丹参能够改善高血压病血瘀证患者血清诱导的内皮功能障碍,其机制可能与激活AMPK有关。
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| 关 键 词： | 丹参 高血压病 血瘀证 AMPK |
Effects of Salvia Miltiorrhiza on Endothelial Dysfunction Induced by Serum of Hypertension Patients with Blood Stasis Syndrome |
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| JIANG Xiaoli,DENG Bo,LIU Bin,ZHANG Shuangwei,ZHANG Jingzhi.Effects of Salvia Miltiorrhiza on Endothelial Dysfunction Induced by Serum of Hypertension Patients with Blood Stasis Syndrome[J].Chinese Archives of Traditional Chinese Medicine,2019(3):638-642. |
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| Authors: | JIANG Xiaoli DENG Bo LIU Bin ZHANG Shuangwei ZHANG Jingzhi |
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| Institution: | (Department of Traditional Chinese Medicine, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China;Guaryzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital of Guangzliou Medical University, Guangzhou 510260, China) |
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| Abstract: | JIANG Xiaoli;DENG Bo;LIU Bin;ZHANG Shuangwei;ZHANG Jingzhi(Department of Traditional Chinese Medicine, the Second Affiliated Hospital of Guangzhou Medical University, Guangzhou 510260, China;Guaryzhou Institute of Cardiovascular Disease, The Second Affiliated Hospital of Guangzliou Medical University, Guangzhou 510260, China) |
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| Keywords: | Salvia miltiorrhiza hypertension blood stasis syndrome AMPK |
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