变形杆菌菌落计数方法的探讨

目的]探讨一种更精确的变形杆菌菌落计数方法.方法]依据变形杆菌的特性,采用SS平板培养和试管证实试验对其进行计数,并用传统的营养琼脂斜面底部冷凝水培养法作参照.结果]SS平板抑制变形杆菌的迁徙性生长,得到单个菌落,有助于准确计数,试管证实试验对变形杆菌进行鉴别确证,排除其他细菌的干扰.结论]新方法的计数结果在数量级上与传统方法相吻合,而且计数结果更加精确.

EXPLORATION ON THE METHOD OF THE COLONY COUNT OF BACILLUS PROTEUS

Objective]To study a new method of counting colony of bacillus proteus.Methods]SS plate culture and tube test were used to screen the bacillus proteus,the traditional agar assay with condensate water on slant bottom was adopted as the control.Results]Single colony was obtained from SS plate culture,which facilitated the colony count,and the bacillus proteus was identified by tube test.Conclusion]It shows that the counting effect of the new method is more precise than that of the traditional.