| 川芎总黄酮提取优化及小鼠体外抗氧化作用 |
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| 引用本文： | 许丽璇,李伟斌,蔡建秀. 川芎总黄酮提取优化及小鼠体外抗氧化作用[J]. 中国医院药学杂志, 2010, 0(18) |
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| 作者姓名： | 许丽璇 李伟斌 蔡建秀 |
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| 作者单位： | 泉州儿童发展职业学院;泉州师范学院化学与生命科学学院; |
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| 基金项目： | 福建省科技厅重点项目资助(编号:2009N0049) |
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| 摘 要： | 目的:研究川芎总黄酮(LCTF)的提取和抗氧化作用。方法:总黄酮的提取采用微波提取和索氏提取2种方法,设置不同的因素水平,从而确定最佳提取方案;抗氧化研究采用总黄酮对血清抗活性氧能力的影响,对小鼠肝匀浆生成MDA的影响,对脂肪氧合酶活性的影响,总黄酮对H2O2诱导小鼠氧化溶血的影响。结果:微波提取总黄酮最佳因素提取频率为530W,提取时间为20min,70%乙醇,液固比为15∶1,得率为1.008%。抗氧化作用表明,2.00g.L-1LCTF对脂肪氧合酶的粗酶活性抑制率超过50%;LCTF在0.5～10.0g.L-1范围内,具有较强的体外抗活性氧能力,并能减少小鼠肝匀浆MDA的生成,可抑制H2O2诱导的小鼠红细胞溶血。结论:微波提取LCTF得率较高,且LCTF具有较强的抗氧化能力,并与总黄酮溶液的浓度具有剂量-效应关系。
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| 关 键 词： | 川芎 总黄酮 提取工艺优化 抗氧化性 |
Study on the optimization of extraction technology and the in vitro anti-oxidative effect of total flavonoids from Ligusticum chuanxiong Hort |
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| XU Li-xuan,LI Wei-Bin,CAI Jian-Xiu. Study on the optimization of extraction technology and the in vitro anti-oxidative effect of total flavonoids from Ligusticum chuanxiong Hort[J]. Chinese Journal of Hospital Pharmacy, 2010, 0(18) |
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| Authors: | XU Li-xuan LI Wei-Bin CAI Jian-Xiu |
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| Affiliation: | XU Li-xuan1,LI Wei-Bin2,CAI Jian-Xiu2(1.Quanzhou Children Development Vocational College,Fujian Quanzhou 362000,China,2.School of Chem.&Bioscience,Quanzhou Normal University,China) |
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| Abstract: | OBJECTIVE:To study the optimization of extraction technology and the in vitro anti-oxidative effect of total flavonoids from Ligusticum chuanxiong Hort.(LCTF).METHODS:The extraction of total flavanone from Ligusticum wallichii was used with the microwave extraction and the Soxhlet extraction two methods,established the different factor level,thus determined the optimum extraction plan.The anti-oxidative effects of the total flavanone were observed with the influence on the ability of anti-active-oxygen of s... |
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| Keywords: | Ligusticum chuanxiong Hort. total flavonoids extraction technology optimization anti-oxidative |
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