三磷酸腺苷对人胃癌细胞恶性表型的去恶化作用

目的 观察三磷酸腺苷（ＡＴＰ）对人胃癌Ｍ１７细胞系增殖和分化的影响。方法 应用三磷酸腺苷作用于人胃癌Ｍ１７单细胞克隆亚系，每日记数细胞和计算抑制率，用扫描电镜观察Ｍ１７细胞膜表面微绒毛变化，罗氏黄荧光染料传输法观察细胞间隙连接通讯功能的状况，纽蛋白和微丝蛋白双标记法观察微丝骨架和粘着斑组装的变化。结果 （１）ＡＴＰ对人胃癌细胞的增殖有明显的抑制作用，加药处理４天后增殖的抑制率可达８０％以上。（２）

Effect of ATP on malignant phenotype of a human gastric cancer cell line

OBJECTIVE: To study the effect of ATP on proliferation and differentiation of a human gastric cancer cell line. METHODS: M17, a subclone of human gastric cancer cell line MGC-803, was treated in vitro with ATP(0.23 mg/ml). After 48 hr, the following parameters were examined. (1) Proliferation of cells by daily cell counting. (2) Microvilli of the cells were observed by scanning EM. (3) Gap junction intercellular communication was examined by mechanical scraping and Lucifer dye transfer. (4) Microfilament of the cytoskeleton was studied with fluorescin-labeled phalloidin. (5) Vinculin adhesion plague was stained immunocytochemically with monoclonal antibodies. RESULTS: ATP significantly inhibited the in vitro growth of M17 cells. The ATP-treated M17 cells showed disappearance of microvilli on the cell surface, increased gap junction intercellular communication, improved organization of microfilament bundles and reappearance of vinculin adhesion plagues. CONCLUSION: ATP not only inhibits proliferation but also induces differentiation of a human gastric cancer cell line with reversion from malignant toward normal phenotype. The parameters examined could serve as useful biomarkers of human gastric cancer.