人绒癌裸鼠皮下移植瘤模型的放射免疫显像

目的 探讨放射免疫显像对人绒癌裸鼠皮下移植瘤的显像效果。方法 应用＾１３１Ｉ标记的小鼠抗ｈＣＧ单克隆抗体，对人绒癌裸鼠皮下移植瘤模型进行放射免疫显像，以正常小鼠ＩｇＧ作为对照，观察放射免疫显像效果，并测定不组织中的放射性活度，计算肿瘤／非肿瘤放射活性比（Ｔ／ＮＴ比值）。结果 在注入标记抗体后２４小时，移植瘤部位即可见放射性浓聚，并随时间的推移而浓聚增强，７２－９６小时后肿瘤可以清晰显像，可显像的最

Radioimmunodetection of human trophoblastic cancer xenograft in nude mice

OBJECTIVE: To study the efficiency of radioimmunodetection to locate xenograft of human trophoblastic cancer in nude mouse. METHODS: Radioimmunodetection was performed with a cocktail of 131I-labeled mouse anti-hCG monoclonal antibodies to image xenogaft of human trophoblastic cancer in nude mice. Normal mouse IgG used to treat mice bearing trophoblastic cancer xenograft was used as control. Radioactivity in different tissues was measured and the tumor/non-tumor(T/NT) ratio was calculated. RESULTS: The accumulation of radioactivity in the xenograft could be recognized as early as 24 hours after the injection of the radiolabeled anti-hCG antibodies. Radioactivity accumulation became increasingly evident with time. At 72-96 hours after injection of the radiolabled antibodies, the xenograft could be clearly shown. The minimal size of the xenograft with demonstrable radioactively was 0.8 cm in diameter. The T/NT ratio increased with time and was obviously higher than that in mice treated with normal mouse IgG. CONCLUSION: Radioimmunodetection can efficiently locate human trophoblastic cancer xenograft in nude mice.