高浓度EB病毒感染人永生化上皮细胞Hacat系

目的探讨EB病毒感染上皮细胞的机制。方法大规模培养绒猴淋巴细胞B958系,从中提取并浓缩EB病毒,用胎儿脐带血淋巴细胞滴定后,以高浓度EB病毒感染人永生化上皮细胞Hacat系。提取Hacat细胞基因组DNA,PCR扩增EB病毒基因组特异性核酸序列BamHIw片断,Southernblotting及原位杂交验证感染结果。结果PCR、Southerblotting及原位杂交结果证实高浓度EB病毒能够感染Hacat细胞,但是感染率非常低。结论EBV对上皮细胞存在CR2或多聚IgA受体介导之外的未知感染途径。

Infection of immortalized human epithelial cell line Hacat with high-concentration Epstein-Barr virus

Objective To study the mechanism by which Epstein-Barr (E B) virus infects human epithelial cells. Methods Large-scale culture of marmoset l ymphocytes B958 was carried out to extract and condense EB virus therein. Titra ted by lym-phocytes from fetal umbilical blood, the EB virus of high concentrati on was used to infect immortalized human epithelial cell line Hacat, followed b y genomic DNA extraction from the Hacat cells and amplification of the special D NA sequence Bam HIw fragments of EBV genomic DNA by PCR. Southern blotting and in situ hybridization were employed to confirm the result of PCR. Results The r esults of PCR, Southern blotting and in situ hybridization all indicated that hi gh-concentration EBV could infect Hacat cell line, but the rate of infection wa s rather low. Conclusion There is an unknown pathway of infection for EBV entry into the epithelial cells other than the mediation by CR2 or pIgR.