乙肝病毒携带产妇乳汁、唾液乙肝病毒标志物检测结果分析

目的:探讨乙肝病毒携带产妇乳汁、唾液中乙肝病毒的携带情况,以指导正确进行母婴喂养和母婴接触。方法:用ELISA法检测32例携带乙肝病毒产妇的血液、乳汁、唾液乙肝病毒标志物;用荧光定量PCR法检测其中25例孕产妇血清的HBV-DNA含量。结果:乙肝病毒携带产妇的乳汁HBsAg阳性率为40.6%,唾液HBsAg阳性率为37.5%,两者没有显著性差异(P>0.05);血HBeAg阳性产妇乳汁和唾液HBsAg阳性率、血HBV-DNA阳性率均比HBeAg阴性者高(P<0.05);部分HBeAg阴性乙肝病毒携带产妇血清HBV-DNA阳性,乳汁和唾液中也能检出HBsAg。结论:①血HBeAg阳性产妇乳汁、唾液潜在传染性高,建议采用人工喂养,最好适当进行母婴隔离。②血HBeAg阴性乙肝病毒携带产妇仍有传染HBV的可能,建议检测血HBV-DNA含量,以指导正确进行哺乳及母婴接触。

Research on hepatitis B virus markers in breast milk and saliva of hepatitis B virus carried mothers

Objective:To research the hepatitis B virus(HBV) carrying rate from the breast milk and saliva of the hepatitis B virus-carried mothers and to give an advice for breast-feeding and contact between mothers and infants.Methods:HBV marker in serum,breast milk and saliva of HBV carried-mothers was tested with the ELISA;HBV DNA in serum was tested with the real-time quantitative polymerase chain reaction.Results:The detective rate of HBsAg in the breast milk and saliva from HBV carried mothers was 40.6% and 37.5% respectively.The detective rate of HBV DNA in serum and HBsAg in breast milk,saliva of HBeAg positive-mothers were higher than HBeAg negative's;HBV DNA in serum also could be detected in some HBV carried mothers of HBeAg negative.Conclusion:The possible transmission by breast milk and saliva is higher in HBeAg positive-mothers than HBeAg negative's.Breast-feeding and contact between mothers and infants are discovered.To direct the breast-feeding and contact between mothers and infants correctly,HBV DNA detection should be suggested.