VEGF-D/VEGFR-3在喉癌中的表达及与淋巴结转移的关系

目的研究血管内皮生长因子D和血管内皮生长因子受体3(VEGF-D/VEGFR-3)在喉癌、癌周组织中的的表达情况及其在喉癌的发生和淋巴结转移中的作用,分析其与喉癌的临床病理参数的关系及相关性。方法选择46例喉癌患者的手术切除标本、14例癌周组织标本,采用免疫组化SP法,观察VEGF-D/VEGFR-3在喉癌中表达的相关性及与患者的年龄、性别、临床TNM分期、病理类型、肿瘤的原发部位和淋巴结转移的关系。结果①喉癌组织中VEGF-D/VEGFR-3表达的阳性率明显高于癌周组织,两组间比较差异有统计学意义(P<0.01)。②VEGF-D/VEGFR-3阳性表达率与肿瘤的分化程度、淋巴结转移和临床TNM分期密切相关(P<0.05),而与年龄、肿瘤原发部位及性别无关(P>0.05)。结论 VEGF-D可作为喉癌预后的一种新指标,阻断VEGF-D/VEGFR-3通路将有望成为喉癌淋巴结转移预防和治疗的新靶点。

Expression of VEGF-D/VEGFR-3 in Laryngeal Carcinoma and Their Relationship with Lymphatic Metastasis

Objective To explore the expression of vascular endothelial growth factor D(VEGF-D) and vascular endothelial growth factor receptor 3(VEGFR-3) in laryngeal carcinoma and adjacent tissues and their role in the occurrence and lymphatic metastasis of the cancer,and analyze the relativity and relationship of them with the clinical parameter of the laryngeal carcinoma.Methods The expression of VEGF-D and VEGFR-3 were detected by using immunohistochemistry technique in 46 cases of laryngeal carcinoma and 14 cases of adjacent tissue of them.The correlation between the expression of VEGF-D/VEGFR-3 and the occurrence of laryngeal carcinoma was analyzed,and their relationship with age,gender,surgical-pathological stages,pathological grades,metastasis of lymph nodes was discussed.Results ①the positive rate of VEGF-D/VEGFR-3 in laryngeal carcinoma was higher than that in adjacent tissue,the difference was significant between the two groups(P<0.01).②The high expression of VEGF-D and VEGFR-3 in laryngeal carcinoma was closely correlated with the histological type,metastasis of lymph nodes and surgical-pathological stages.but no association was observed with age,the tumor original position and gender(P>0.05).Conclusion VEGF-D could come to be a new index to estimate the prognosis of laryngeal carcinoma and blocking the signal pathway in the expression of VEGF-D/VEGFR-3 may become a new approach to anti-lymphatic metastasis of laryngeal carcinoma.