| 海洋中度嗜盐菌whb36的分离、鉴定和生物活性研究 |
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| 引用本文： | 陈雷,王光玉,张允斌,卜同,刘明,林秀坤.海洋中度嗜盐菌whb36的分离、鉴定和生物活性研究[J].中国海洋药物,2010,29(2). |
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| 作者姓名： | 陈雷 王光玉 张允斌 卜同 刘明 林秀坤 |
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| 作者单位： | 中国科学院海洋研究所实验海洋生物学重点实验室;哈尔滨工业大学（威海）海洋学院;中国科学院研究生院; |
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| 基金项目： | 中国科学院知识创新工程项目(KSCX-YW-104,KZCX2-YW-209) |
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| 摘 要： | 目的从海洋中度嗜盐细菌中发现具有抗菌和抗肿瘤活性的物质。方法从盐场的底泥和海水中分离嗜盐细菌,采用抗菌模型和细胞毒模型进行活性筛选,对活性较强的菌株whb36进行形态学和生理生化特性研究,测定其16S rRNA序列并通过同源性比对进行系统发育分析。结果菌株whb36为中度嗜盐菌;whb36与Halomonas ventosae在形态和生理生化特征方面最接近,16S rRNA序列相似性为99%。whb36的粗提物具有较强的抗菌和抗肿瘤活性。结论中度嗜盐细菌whb36可以提供新的活性物质。
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| 关 键 词： | 中度嗜盐菌 分离 鉴定 抗菌活性 抗肿瘤活性 |
Isolation and identification of marine moderately halophilic bacterium whb36 and screening of its biological activity |
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| CHEN Lei,WANG Guang-yu,ZHANG Yun-bin,BU Tong,LIU Ming,LIN Xiu-kun.Isolation and identification of marine moderately halophilic bacterium whb36 and screening of its biological activity[J].Chinese Journal of Marine Drugs,2010,29(2). |
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| Authors: | CHEN Lei WANG Guang-yu ZHANG Yun-bin BU Tong LIU Ming LIN Xiu-kun |
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| Institution: | 1.Key Laboratory of Experimental Marine Biology;Institute of Oceanology;Chinese Academy of Sciences;Qingdao 266071;China;2.College of Marine Science;Harbin Institute of Technology at Weihai;Weihai 264209;3.Graduate School;Beijing 100039;China |
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| Abstract: | Objective To find the novel antimicrobial and antitumor substances from marine moderately halophilic bacteria.Methods The halophilic bacteria were isolated from sediment and saline water collected from Weihai solar saltern of Shandong province.The antimicrobial activity and cytotoxic activity were screened.The identification and phylogenetic analysis of the isolated active strain whb36 were determined by their morphological characteristics,physiological and biochemical tests and molecular identification bas... |
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| Keywords: | moderately halophilic bacteria isolation identification antimicrobial activity antitumor activity |
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