采用通用引物进行PCR检测宫颈中人乳头瘤病毒的基因

采用可检测九个型别人乳头瘤病毒（ＨＰＶ）的通用引物进行多聚酶链反应，在４７份宫颈糜烂患者的宫颈细胞标本中检出了ＨＰＶ阳性的标本２３份。在这２３份阳性标本中ＨＰＶ１６型特异性引物进一步用ＰＣＲ进行扩增，检出ＨＰＶ１６型阳性者１０份，在２４份ＨＰＶ阴性的标本中用ＨＰＶ１６型特异性引物未检出ＨＰＶ１６阳性的标本。结果表明：用通用引物进行ＰＣＲ检测人乳头瘤病毒的感染，方法简便、灵敏、经济，特别适合于ＰＣＲ

Detection of HPV-DNA in Cervical Tissues by Polymerase Chain Reaction with General Primers

Polymerase chain reaction (PCR) with general primers capable of amplify-ing 9 types of human papillomavirases (RPVs)was used. Twenty-three cases of 47 cervical tissue specimens from patients with cervical erosion were positive for HPV-DNA (49%).In these 23 spacimens ,HPV16 was not found in any of the 24 HPV-negatine specimens. These results indicated that PCR using general primers to detect HPVs is simple .sensitive and eco-nomic.and particularly suitable for detection of HPV infection.