多参数流式细胞术检测41例维吾尔族骨髓增生异常综合征患者粒、单核细胞免疫表型的临床意义

目的：探讨维吾尔族骨髓增生异常综合征（MDS）患者粒、单核细胞免疫表型特点及其在诊断分型和预后中的临床意义。方法应用多参数流式细胞术（flow cytometry ，FCM ），对41例MDS患者和30例非MDS（non-MDS）患者粒、单核细胞免疫标志的表达率进行检测。结果 MDS与 non-MDS相比，粒细胞群比例显著降低（P＝0．021），单核细胞比例显著升高（P＝0．000）；粒、单核细胞群比例在MDS亚型间均无显著差异（P＞0．05）；CD10、CD11b、CD15、CD16和CD66d在成熟粒细胞群表达显著增高（P值分别为：0．012、0．027、0．016、0．005和0．024），CD33、CD13、HLA-DR、CD56及CD117表达均无显著差异（P值分别为0．058、0．337、0．649、0．096和0．081），CD10在RAEB-II型表达显著低于RAEB-I型（P＝0．001），CD13和 HLA-DR在RC-MD型表达显著低于RAEB-I和RAEB-II型（P分别为0．013、0．005和0．018、0．003），其余抗原亚型间比较均无显著差异（P＞0．05）；CD15、CD33和CD64在单核细胞群表达均显著升高（P值分别为：0．001、0．007和0．000），CD7、HLA-DR、CD14、CD13、CD61和CD56表达均无显著差异（ P值分别为：0．075、0．066、0．408、0．153、0．137和0．322），所有抗原亚型间比较均无显著差异（ P＞0．05）。结论多参数FCM检测能够发现维吾尔族MDS患者粒、单核细胞异常免疫表型特点，为临床上对MDS患者诊断分型和预后评价提供更多有价值的客观信息。

Clinical Significance of the Immunophenotypic Features of granulocytes and monocytes in 41 Uyghur Patients with Myelodysplastic Syndromes by Multiparametric Flow Cytometry

Objective To research the characteristics of immunophenotype of granulocytes and monocytes in 41 Uyghur patients with Myelodysplastic syndrome(MDS) and its clinic significance .Methods The expressions of antigens of granulocyte and mono-cyte population in 41 patients with MDS were detected by multiparametric flow cytometry .Results Comparing with the non-MDS group ,the percentage of granulocyte was significantly decreased (P= 0 .021) ,the proportion of monocyte population was signifi-cantly higher in the MDS group (P=0 .000) .The proportion of nucleated erythrocyte population was no significant differences in RCMD ,RAEB-I and RAEB-II (P>0 .05) .The expressions of CD10 ,CD11b ,CD15 ,CD16 and CD66d on granulocytes were much higher than non-MDS group(P=0 .012 ,P=0 .027 ,P= 0 .016 ,P= 0 .005 ,P= 0 .024 ,respectively);there were no significantly differences of the expressions of CD33 ,CD13 ,HLA-DR ,CD56 and CD117(P=0 .058 ,P=0 .337 ,P=0 .649 ,P=0 .096 ,P=0 .081 , respectively );the expression of CD10 in RAEB-II was decreased significantly than RAEB-I(P=0 .001);the expressions of CD13 and HLA-DR in RCMD were significantly decreased than RAEB-I and RAEB-II(P=0 .013 ,P=0 .005 and P=0 .018 ,P=0 .003 , respectively) .Other expressions of antigens were no significantly differences among the subtypes of MDS (P>0 .05) .The expres-sions of CD15 ,CD33 and CD64 on monocytes were significantly higher than non-MDS group(P=0 .001 ,P=0 .007 ,P=0 .000 ,re-spectively) ,however ,there were no significantly differences of the expressions of CD7 ,HLA-DR ,CD14 ,CD13 ,CD61 and CD56 on monocytes(P=0 .075 ,P=0 .066 ,P=0 .408 ,P=0 .153 ,P=0 .137 ,P=0 .322 ,respectively ) .Expressions of all antigens were no significantlydifferencesamongthesubtypesofMDS(P>0.05).Conclusion Multiparametricflowcytometry (FCM)canrevealthe characteristics of immunophenotypic abnormalities of granulocytes and monocytes in Uyghur patients with MDS and provide more valuable ,objective information for the diagnosis and prognosis .