| 不同剂量U0126抑制低频磁刺激引起星形胶质细胞迁移的实验研究 |
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| 引用本文： | 李 哲,方征宇,黄晓琳.不同剂量U0126抑制低频磁刺激引起星形胶质细胞迁移的实验研究[J].中国康复医学杂志,2010,25(3):195-199. |
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| 作者姓名： | 李 哲 方征宇 黄晓琳 |
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| 作者单位： | 1. 华中科技大学附属同济医院,武汉,430030
2. 华中科技大学附属同济医院康复医学科 |
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| 基金项目： | 国家自然科学基金资助项目（30801220） |
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| 摘 要： | 目的:研究不同剂量U0126对低频磁刺激促进星形胶质细胞迁移作用的影响,以选择合适的阻滞剂量。方法:24只SD大鼠按U0126剂量被随机分为对照组(0mg/kgU0126,n=6)、低剂量组(0.1mg/kgU0126,n=6)、中剂量组(0.2mg/kgU0126,n=6)、高剂量组(0.4mg/kgU0126,n=6),四组的刺激参数都为频率1Hz,强度1.52T,刺激量为30脉冲;均采用溴已锭注射入脊髓左侧背索复制局灶性的脊髓损伤模型。刺激后第14天,大鼠被处死,采用图像分析系统观察GFAP、MAP-2和ERK1/2的表达及脊髓损伤空洞体积的变化。结果:随着U0126剂量的增加,在第14天时空洞的体积逐渐增大,GFAP、ERK1/2的阳性表达逐渐减弱,与对照组相比具有显著性差异(P0.05),U0126中剂量组和高剂量组作用则无明显差异(P0.05);病灶区域MAP-2均呈阴性表达。结论:不同剂量的U0126可翻转磁刺激引起的星形胶质细胞的迁移,0.2mg/kg是较合适的剂量。
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| 关 键 词： | 低频 磁刺激 星形胶质细胞 迁移 |
| 收稿时间： | 2009/12/21 0:00:00 |
The effects of U0126 of different doses on the ability of low frequency magnetic stimulation promoting astrocyte migration |
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| LI Zhe,FANG Zhengyu,HUANG Xiaolin.The effects of U0126 of different doses on the ability of low frequency magnetic stimulation promoting astrocyte migration[J].China Journal of Rehabilitation Medicine,2010,25(3):195-199. |
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| Authors: | LI Zhe FANG Zhengyu HUANG Xiaolin |
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| Institution: | LI Zhe,FANG Zhengyu,HUANG Xiaolin Department of Rehabilitation,Tongji Hospital,Huazhong University of Science , Technology,Wuhan,430030 |
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| Abstract: | Objective:To investigate the effects of U0126 of different doses on the ability of low frequency magnetic stimulation promoting astrocyte migration and to select suitable dose of U0126. Method:Twenty-four adult healthy SD rats were selected to receive injection of 0.5ml of 1% ethidium bromide (EB) in PBS into the dorsal spinal cord funiculus on the left side at T10-11 level to make located spinal cord injury models and randomly divided into four groups.The four groups were exposed to magnetic stimulation (1... |
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| Keywords: | low frequency magnetic stimulation astrocyte migration |
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