特发性血小板减少性紫癜患者外周血调节性T细胞的研究

目的研究特发性血小板减少性紫癜（ITP）患者外周血CD4^＋CD25^＋调节性T细胞（Tr细胞）的变化，探讨其在ITP发病机制中的作用。方法分别收集35例ITP患者及健康对照组外周抗凝静脉血，分离纯化T淋巴细胞。应用藻红蛋白（PE）标记的抗CD4单抗及异硫氰酸荧光素（FITC）标记的抗CD25单抗，作双色流式细胞术，分析ITP患者外周血CD4^＋CD25^＋调节性T细胞百分率。结果ITP组外周血中Tr细胞明显低于健康对照组（P〈0．05），Tr细胞占CD4^＋T细胞的比例也低于健康对照组（P〈0．05）。结论ITP患者外周血存在细胞免疫功能失调，Tr细胞数量减少可能与ITP的发病机制有关。

The research of peripheral blood regulatory T cell in patients with idiopathic thrombocytopenic purpura

Objective To investigate the change of peripheral blood CD4+CD25+ regulatory T cells (Tr) in patients with idiopathic thrombocytopenic purpura (ITP), and to analyze its role in the pathogenesis of ITP.Methods Anticoagulated venous blood was collected from ITP patients (ITP group,n=35) and healthy controls (healthy control group,n=35). T lymphocytes were isolated and purified with human CD3+ T cell enrichment columm. The percentage of peripheral blood CD4+CD25+ regulatory T cells was detected by immunofluorescence staining (PE-anti-CD4 monoclone antibody and FITC-CD25 monoclone antibody) and bicolor flow cytometry by CD25/CD4 gating.Results The number and constituent ratio of CD4+CD25+T cell were significantly lower in ITP group than those of healthy control group (P<0.05).Conclusion There is peripheral blood celluar immunological function disorder in ITP patients, and decrease of CD4+CD25+ T cell population may be involved in the pathogenesis of ITP.