柴胡皂苷a、d在宁夏栽培与野生柴胡中含量的比较研究

目的建立高效液相色谱-蒸发光散射检测法,对宁夏栽培与野生柴胡中柴胡皂苷a、d的含量进行比较研究。方法 Agilent ZORBAX XDB-C18色谱柱（250 mm×4.6 mm,5μm）,以乙腈-水（43∶57）为流动相,流速1.0 ml/min,柱温：30℃,Agilent ELSD 2000ES检测器,漂移管温度：97℃,气速：2.6 L/min。结果柴胡皂苷a、d的线性范围分别是0.2002-8.008μg（r=0.999 5）,0.261-10.45μg（r=0.998 5）;平均回收率分别为97.4%、97.5%;RSD分别为1.5%、2.5%。宁夏栽培与野生柴胡中柴胡皂苷a、d的含量之和范围在0.92%-1.61%。结论宁夏栽培柴胡中柴胡皂苷a、d的含量之和与野生柴胡基本一致。本法简便、准确、重复性好,可作为柴胡药材质量检测的方法。

Comparative study of saikosaponins a,d in wild and cultured Bupleurum in Ningxia

Objective To establish a method of HPLC-ELSD for determination of saikosaponins a,d in wild and cultured Bupleurum in Ningxia.Methods An Agilent ZORBAX XDB-C18（250 mm×4.6 mm,5 μm） column was used at 30 ℃,acetonitril-water（43：57） was used as the mobile phase.The flow rate was 1.0 ml/min.Agilent ELSD 2000ES detector as the detector.The drift tube temperature was 97 ℃,the gas flow rate was 2.6 L/min.Rusults The linear ranges of saikosaponin a,d were 0.2002-8.008 μg（r=0.9995）,0.261-10.45 μg（r=0.9985）.The average recoveries were 97.4%,97.5% for saikosaponins a,d,respectively.The ranges of the content of saikosaponins a and d in wild and cultured Bupleurum in Ningxia were 0.92%-1.61%.Conclusion The total content of saikosaponins a and d in cultured Bupleurum in Ningxia are basically identical with wild Bupleurum.This method is simple,sensitive and reliable.It can be used for the quality control of Bupleurum.