PKC信号传导对ITP患儿外周血T细胞凋亡及Fas/FasL蛋白表达的影响

目的探讨蛋白激酶C(PKC)信号传导对ITP患儿外周血T细胞凋亡及其相关蛋白Fas、FasL表达的影响。方法无菌采集ITP患儿及正常健康儿童的外周抗凝血,分离T细胞,并分为三组,第一组为空白对照组,第二组加入PKC的激活剂PMA,第三组加入PMA与抑制剂H-7;在37℃、5%CO2条件下培养24小时,用流式细胞仪检测T细胞的凋亡率及Fas、FasL蛋白的表达。结果加入PMA培养液的正常组,T细胞的凋亡率和Fas、FasL蛋白表达与空白对照比较,差别均无显著性差异(P>0.05);加入PMA培养液的ITP组,T细胞的凋亡率与空白对照比较,差别有统计学意义(P<0.05),与加入PMA培养的正常组比较,差别也有显著性(P<0.05),ITP患儿T细胞Fas的表达与其它各组比较,差别均有统计学意义(P<0.05),而FasL的表达仅与ITP组空白对照相比有统计学意义(P<0.05);同时加入PMA和H-7培养液的ITP组,T细胞的的凋亡率和Fas、FasL蛋白表达与只加入PMA培养液的ITP组比较,差异也有显著性(P<0.05),而与空白对照比较,差别均无显著性(P>0.05)。结论PKC信号传导对ITP患儿T细胞凋亡及其相关蛋白Fas、FasL的表达产生影响,并且可能与ITP的免疫病理机制有关。

Effect of protein kinase C signal transduction on expression of Fas/FasL and apoptosis of T lymphocytes in ITP children

Objective To investigate the effect of protein kinase C(PKC) signal transduction on expression of Fas/FasL and apoptosis in peripheral blood T lymphocytes in ITP children. Methods Peripheral blood T lymphocytes from ITP children and normal heathy children were isolated.Then T cells were divided into three groups(A,B and C)and cultured for 24h under the condition of 37℃,5%CO2.Group A is blank,PMA was put into group B and PMA+H-7 was put in to group C.We detected T cells apoptosic rate and expression of Fas/FasL by FCM. Results T Lymphocyte apoptosic rate:compared with blank control,normal group with PMA culture medium was of no significance (P>0.05),compared with blank control and normal group with PMA culture medium,ITP group with PMA had signi-ficant difference(P<0.05).ITP group with PMA and H-7 culture medium had significant difference(P<0.05)only compared with ITP group with PMA culture medium.Expression of Fas/FasL on T lymphocytes:compared with blank control,normal group with PMA culture medium was of no significance (P>0.05)compared with normal groups and the other ITP groups, Fas expression of ITP group with PMA had significant difference(P<0.05),but FasL expression of ITP group with PMA had significant difference (P<0.05) only compared with the blank contro1 in ITP groups. ITP group with PMA and H-7 culture medium had significant difference(P<0.05)only compared with ITP group with PMA culture medium,but had no significance compared with blank control(P>0.05).Conclusion PKC signal transduction ways can affect T lymphocytes apoptosis and expression of Fas/FasL in ITP children,besides,it can relate to immunity pathogenesis of ITP.