甲状腺功能亢进症患者外周血中粒细胞集落刺激因子及其受体表达的意义

目的:观察甲状腺功能亢进(简称甲亢)患者外周血中粒细胞集落刺激因子(G-CSF)和其受体(G-CS-FR)的表达及临床意义。方法:采用双抗体夹心ELISA法和G-CSFR单克隆抗体及流式细胞技术,检测63例初发甲亢患者、22例应用抗甲状腺药物(ATD)治疗控制的甲亢患者及43例正常人外周血中G-CSF和G-CSFR的表达水平,分析粒细胞计数与G-CSF水平及G-CSFR表达的关系。结果:G-CSFR表达率、白细胞总数及中性粒细胞绝对值三组间均无明显差异(P>0.05)。初发甲亢组和甲亢控制组血清G-CSF水平均明显高于正常对照组(P<0.01和P<0.05);各组中G-CSF和G-CSFR与白细胞计数之间无明显的相关性(P>0.05)。结论:甲亢患者外周血中G-CSF水平是升高的,可能与机体免疫反应有关。

Clinical Significance of Expression of Granulocyte Colony-Stimulating Factor and Its Rceptors in Peripheral Blood of Patients With Hyperthyroidism

Objective: To observe the expression of Granulocyte clony-stimulating factor (G-CSF) and its receptor(G-CSFR)in peripheral blood of patients with hyperthyroidism and explore its clinical significance. Methods: Fourty-three normal controls, 63 novel patients of hyperthyroidism and 22 patients with antithyroid drug.(ATD) therapy were investigated with enzyme-linked immunosorbent assay(ELISA), monoclonal antibody and flow cytometry, the relationship between the expression of G-CSF,G-CSFR level and granulocyte in peripheral blood were analyzed. Results: There were no significant differences in G-CSFR expression rates, numbers of leukocyte and neutrophil between three groups(P> 0.05). The Levels of G-CSF in serum of patients with hyperthyroidism was significantly higher than that of normal control (novel group P< 0.01,ATD therapy group P< 0.05). There was no significant correlation in G-CSFR expression rate and G-CSF levels with number of neutrophil between all groups(P> 0.05). Conclusion: The Levels of G-CSF in peripheral blood increased in patients with hyperthyroidism. These results may be related to immune response.