饮水中有机致突物对小鼠体细胞遗传损伤的分子机制研究

通过居家饮水中提取的有机浓缩物亚急性染毒小鼠后,在动物肝、肾、肠组织观察到显微病理及超微病理学变化;各实验组小鼠胸骨骨髓嗜多染红细胞微核率高于阴性对照组。用PCR- SSCP方法可检出高剂量组肝、肾、肠组织DNA p53基因点突变,尤以第7外显子突变频率较高。提示用分子生物学技术研究饮水有机致突物在实验小鼠体内遗传毒性机制的可行性

Study on the molecular mechanism of genetic damages in body cells in mice treated with organic mutants in drinking water

The genetic toxicity of some volatile compounds of chlorinated by products have been determined in animal studies. But little is known at present about the genetic toxicity of nonvolatile matter in drinking water in vivo assay.In a sub acut experiment, the mice were exposed to the organic compounds extracted from in home tap water. The pathologic changes in the liver and kidney tissues were observed under microscope and electron microscope. The frequencies of micronuclei in polychromatic erythrocytes in mice chest bone marrow in the three treated groups were tested. The point mutants in p53 gene of liver, kidney and colon tissues in the high dosage group were examined by PCR SSCP. Especially, the frequencies of mutations in exon 7 of p53 gene in those mice were higher than those in exon 5 of p53 gene. It showed that the study on the molecular genetic toxicity of organic mutants in vivo assay was possible