肝静脉阻断后肝癌、癌周、肺组织及血浆中粘附分子-1的表达

采用EnVisionTM免疫组化方法 ,观察肝癌模型Wistar大鼠肝静脉阻断后肝癌及癌周组织、邻近肝叶、肺组织粘附分子 1 (ICAM 1 )的表达变化 ,采用免疫测定法观察血浆中ICAM 1的变化 ,以探讨肝静脉阻断对肝癌生长、侵袭和转移的影响。发现 :肝静脉阻断后肝癌组织ICAM 1的表达率为 1 8.8% ( 9/4 8) ,较对照组 ( 6 6 .7% ,8/1 2 )明显下调(P <0 .0 1 ) ;癌周组织ICAM 1的阳性表达率为 33.3% ( 1 6 /4 8) ,肝左中叶ICAM 1的阳性表达率为 39.6 % ( 1 9/4 8) ,与对照组比较均未见差异 ,而肺组织ICAM 1的表达率为 70 .8% ( 34 /4 8) ,明显高于对照组 ( 2 5 .0 % ,3/1 2 ) ,P <0 .0 5 )。肝静脉阻断后血浆ICAM 1含量明显高于肝静脉阻断前 (P <0 .0 1 ) ,对照组手术前后血浆ICAM 1含量无明显变化。结果提示肝癌肝静脉阻断可促进癌细胞向远隔器官 (肺组织 )转移。

Expression of ICAM-1 in Liver Cancer,Paracarcinoma and Lung Tissue after Occlusion of Hepatic Vein

A hepatocellular carcinoma model of Wistar rat was constructed to study the expression of ICAM-1 in hepatocellular carcinoma and remote organs and to assess the growth, metastasis and invasion of cancer cell after the occlusion of hepatic vein. The change of expression of ICAM-1 in liver cancer, paracarcinoma tissue, nearby hepatic lobe and lung were measured by EnVision immunohistochemistry and the quantity of ICAM-1 in plasma by immunosurvey. The results showed that ICAM-1 in hepatocellular carcinoma tissue samples significantly downgraded after occulsion of hepatic vein compared with the control group (P<0.01). Positive samples of expression in paracarcinoma tissue (33.3%) and positive sample of expression in the left median lobe of the lever (39.6%) were fewer than the negative samples; while there were fewer positive samples than negative samples in hepatic left median lobe. However, we didn't find obviously discrepancy in contrast to the control group (P>0.05). The expression of ICAM-1 in the lung was higher than the control group (P<0.05). In plasma, the quantity of ICAM-1 extremely increased after the operation (P<0.01). Nevertheless, there wasn't a marked change found in the control group from pre-operation to post-operation. Therefore, we have the conclusion that the metastasis of malignant cell is different in different organs or tissues, and occlusion of hepatic vein accelerates cancer cell's transfer to remote part (lung) of body.