特发性扩张型心肌病与HLA-A*基因的关联研究

目的探讨特发性扩张型心肌病（idiopathic dilated cardiomyopathy，IDC）易感的分子机制和确立一种人类主要组织相容性复合体（HLA—A＊）基因的检测方法。方法采用聚合酶链式反应和顺序特异性引物（PCR—SSP）基因分析方法，对31例特发性扩张型心肌病患者及29例无血缘关系的健康人的HIA—A＊各等位基因及亚基因进行检测分析，并将该方法与其他检测HIA等位基因的方法进行对比。结果HIA—A＊ 03基因与IDC呈正相关（RR=4．697，P〈0．05），其他HIA—A＊各等位基因未见异常。结论HIA—A＊03基因可能是北方汉族人IDC的致病易感基因之一。采用的方法（PCR—SSP）具有快速、简便、敏感、准确和可靠等优点，值得推广。

HLA-A* gene polymorphism in patients with idiopathic dilated cardiomyopathy

Objective In order to study the correlativity between HLA-A*genepolymorphism and idiopathic dilated cardiomyopathy(IDC)and to establish a method for testing HLA-A polymorphism. Methods HLA-A*gene polymorphism in 31 patients with IDC and 29 normal control subjects was analyzed by using the polymerease chain reactionsequence specific primer (PCRSSP)technique. Result The frequency of HLA-A*03 allele was significantly higher in patients than that in the controls,RR of IDC patients was 4.697, P < 0.05. Conclusion These findings further verify that immunogenetics play a predominant pathogenetics role in a subset of IDC patients. Individuals carrying HLA-A*03 alleles may be susceptible gene to IDC. This method is specific, sensitive, simple,rapid and accurate,and could be applied to the routine clinical detective.