| 不同材质及孔径超滤膜对麦冬皂苷类成分的影响 |
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| 引用本文： | 罗瑞芝,张会梅,苏小琴,刘雪,李德坤,周大铮,叶正良,鞠爱春,王辉. 不同材质及孔径超滤膜对麦冬皂苷类成分的影响[J]. 现代药物与临床, 2018, 41(3): 457-461 |
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| 作者姓名： | 罗瑞芝 张会梅 苏小琴 刘雪 李德坤 周大铮 叶正良 鞠爱春 王辉 |
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| 作者单位： | 天津中医药大学, 天津 300190,天津中医药大学, 天津 300190,天津天士力之骄药业有限公司, 天津 300402;天津市中药注射剂安全性评价企业重点实验室, 天津 300402,天津中医药大学, 天津 300190,天津天士力之骄药业有限公司, 天津 300402;天津市中药注射剂安全性评价企业重点实验室, 天津 300402,天津天士力之骄药业有限公司, 天津 300402;天津市中药注射剂安全性评价企业重点实验室, 天津 300402,天津天士力之骄药业有限公司, 天津 300402;天津市中药注射剂安全性评价企业重点实验室, 天津 300402,天津天士力之骄药业有限公司, 天津 300402;天津市中药注射剂安全性评价企业重点实验室, 天津 300402,山东大学齐鲁儿童医院, 山东 济南 250022 |
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| 摘 要： | 目的 考查不同材质及不同孔径的超滤膜对5种麦冬皂苷类成分的影响。方法 以麦冬皂苷C、麦冬皂苷D、麦冬皂苷D''、麦冬皂苷Ra、龙血苷F为考察指标，利用HPLC-UV法测定其超滤前后变化，考察不同材质及孔径的超滤膜对5种麦冬皂苷收率的影响以及各超滤阶段对麦冬皂苷类成分截留率的变化情况。结果 不同材质及不同孔径的超滤膜处理下，麦冬皂苷类成分的收率不同；聚醚砜材质超滤膜对各超滤阶段的麦冬皂苷类成分截留率变化趋势与纤维素材质是一致的。结论 采用100 kDa的聚醚砜超滤膜，可以使麦冬皂苷类成分的收率达到最高，用50%乙醇进行等体积透析，会对麦冬皂苷的收率有较大的改善。
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| 关 键 词： | 超滤 麦冬皂苷 麦冬 收率 截留率 麦冬皂苷C 麦冬皂苷D 麦冬皂苷D'' 麦冬皂苷Ra 龙血苷F |
| 收稿时间： | 2018-01-22 |
Effect on saponins in Ophiopogonjaponicus by ultrafiltration membranes of different materials and cut-off molecular weight |
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| LUO Ruizhi,ZHANG Huimei,SU Xiaoqin,LIU Xue,LI Dekun,ZHOU Dazheng,YE Zhengliang,JU Aichun and WANG Hui. Effect on saponins in Ophiopogonjaponicus by ultrafiltration membranes of different materials and cut-off molecular weight[J]. Drugs & Clinic, 2018, 41(3): 457-461 |
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| Authors: | LUO Ruizhi ZHANG Huimei SU Xiaoqin LIU Xue LI Dekun ZHOU Dazheng YE Zhengliang JU Aichun WANG Hui |
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| Affiliation: | Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China,Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China,Tianjin Tasly Pride Pharmaceutical Co., Ltd., Tianjin 300402, China;Tianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Tianjin 300402, China,Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China,Tianjin Tasly Pride Pharmaceutical Co., Ltd., Tianjin 300402, China;Tianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Tianjin 300402, China,Tianjin Tasly Pride Pharmaceutical Co., Ltd., Tianjin 300402, China;Tianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Tianjin 300402, China,Tianjin Tasly Pride Pharmaceutical Co., Ltd., Tianjin 300402, China;Tianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Tianjin 300402, China,Tianjin Tasly Pride Pharmaceutical Co., Ltd., Tianjin 300402, China;Tianjin Key Laboratory of Safety Evaluation Enterprise of Traditional Chinese Medicine Injections, Tianjin 300402, China and Qilu Children''s Hospital of Shandong University, Shandong 250022, China |
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| Abstract: | Objective To investigate the effect of the ultrafiltration membrane with different materials and different sizes on five saponins of Ophipogon japonicas. Methods Using Ophipogonin C, ophipogonin D, ophipogonin D'', ophipogonin Ra, and dracaenoside F as index, determine the change of the content before and after the ultrafiltration membrane to measure the effect on the yield of ophiopogonins by HPLC-UV, observe the influence of different materials and pore sizes of ultrafiltration membrane on yields of 5 kinds of saponins. Results The yield of ophiopogonins from Radix Ophiopogonis was different under different material and pore size. The variation trend of the retention rate of ophiopogonins in the ultrafiltration stage was consistent with that of cellulose. Conclusion Using 100kDapolyethersulfone ultrafiltration membrane will have the highest yield of ophiopogonins. Using 50% ethanol for equal volume dialysis can greatly improve the yield of Ophiopogonjaponicus saponin. |
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| Keywords: | ultrafiltration ophiopogonin Ophiopogonjaponicus yield interception rate ophipogonin C ophipogonin D ophipogonin D'' ophipogonin Ra dracaenoside F |
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