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TCR function analysis using a novel system reveals the multiple unconventional tumor-reactive T cells in human breast cancer-infiltrating lymphocytes |
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| Authors: | Satoshi Yamaguchi Hiroshi Hamana Kiyomi Shitaoka Kenta Sukegawa Takuya Nagata Abdul Hayee Eiji Kobayashi Tatsuhiko Ozawa Tsutomu Fujii Atsushi Muraguchi Kazuyuki Tobe Hiroyuki Kishi |
| Affiliation: | 1. Department of Immunology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan Department of First Internal Medicine, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan These authors contributed equally to this work.;2. Department of Immunology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan;3. Department of Immunology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan Department of Immunology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Hiroshima, Japan;4. Department of Immunology, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan Department of Surgery and Science, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan Niigata Medical-Care-Cooperative Kido-Hospital, Niigata, Japan;5. Department of Surgery and Science, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan Toho University Ohashi Medical Center, Tokyo, Japan;6. Department of Surgery and Science, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan;7. Department of First Internal Medicine, Faculty of Medicine, Academic Assembly, University of Toyama, Toyama, Japan |
| Abstract: | Tumor-infiltrating lymphocytes (TILs) are a potent source for obtaining tumor-reactive T cell receptors (TCRs). Although comprehensive methods to analyze the TCR repertoire in TILs have been reported, the evaluation system for TCR-reactivity to endogenously expressed antigen in tumor cells remains laborious and time consuming. Consequently, very limited numbers of TCRs in TILs have been analyzed for their reactivity to tumor cells. In this study, we developed an efficient evaluation system for TCR function designated c-FIT (c omprehensive f unctional i nvestigation of T CRs) to analyze TCR reactivity. The c-FIT system enabled us to analyze up to 90 TCRs for their reactivity to tumor cells by a single assay within a month. Using c-FIT, we analyzed 70 TCRs of CD8+ TILs derived from two breast cancer patients and obtained 23 TCRs that reacted to tumor cells. Surprisingly, although two TCRs were HLA class I-restricted, the remaining 21 TCRs were non-HLA-restricted. Thus, c-FIT can be applied for monitoring multiple conventional and unconventional antigen-specific killer T cells in TILs, leading to the development of new designs for more effective T-cell-based immunotherapies. |
| Keywords: | breast cancer CD8 T cells TCR TCR repertoire tumor immunology |