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确证TRFIA检测乙肝病毒表面抗体结果的研究
引用本文:吉飞跃,钱开成. 确证TRFIA检测乙肝病毒表面抗体结果的研究[J]. 标记免疫分析与临床, 2011, 18(1): 33-35
作者姓名:吉飞跃  钱开成
作者单位:南通大学附属海安医院检验科;
摘    要:
建立简易时间分辨免疫荧光法(TRFIA)检测乙型肝炎病毒表面抗体(抗-HBs)的中和试验方法,验证TRFIA检测结果.收集乙型肝炎病毒表面抗原(HBsAg)异常值血清,确定TRFIA中和抑制试验中和比为0.25-1.26ng/mL∶1mIU/mL,对抗-HBs的定量结果进行确认.结果显示,使用TRFIA定量检测的结果与...

关 键 词:乙型肝炎病毒表面抗体  中和抑制试验  时间分辨免疫荧光法

Detection of Hepatitis B Surface Antigen and Antibody by TRFIA
JI Fei-yue,QIAN Kai-cheng. Detection of Hepatitis B Surface Antigen and Antibody by TRFIA[J]. Labeled Immunoassays and Clinical Medicine, 2011, 18(1): 33-35
Authors:JI Fei-yue  QIAN Kai-cheng
Affiliation:JI Fei-yue,QIAN Kai-cheng(Department of Clinical Laboratory,Nantong University Haian affiliated Hospital,Nantong 226600,China)
Abstract:
To establish a simple neutralization test to detect hepatitis B surface antigen and antibody(anti-HBs) by TRFIA.The serum samples with outlier serum of heptatitis B surface antigen(HBsAg) were collected,and the neutralization inhibition test was carried out to confirm the quantitative result of anti-HBs which analysis by TRFIA.The results showed that the coincidence rate of anti-HBs analysis by TRFIA quantitative test and neutralization inhibition test was 100%.The neutralization inhibition test could be us...
Keywords:Hepatitis B surface antibody  Neutralization inhibition test  Time resolved fluoroimmunoassay  
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