睾酮对自由基损伤的海马神经元神经保护作用中细胞内Ca2+变化

目的：观测睾酮对自由基损伤的海马神经元神经保护用中是否有细胞内Ca2＋变化。方法：原代培养海马神经元,暴露于自由基并损伤后,分为对照组、不同浓度过氧化氢组、睾酮组;利用荧光探针进行标记,激光共聚焦显微镜下观察加入睾酮前后细胞内Ca2＋变化。结果：以100μM和200μM H2O2组引起细胞内Ca2＋增加最为明显;与对照组相比,Fi值变化差异有统计学意义（P〈0.05）;预先加入睾酮后再暴露于100μM H2O2与单独暴露于相同浓度H2O2组相比,细胞内Ca2＋荧光比值明显降低,二者差异有统计学意义（P〈0.05）。结论：自由基损伤能引起原代培养海马神经元内Ca2＋含量升高,睾酮能明显减轻由于自由基损伤引起的细胞内Ca2＋变化。

Intracellular Calcium Changes of Free Radical Exposed Hippocampal Neurons after Administration of Testosterone

Objective： To investigate whether or not the intracellular calcium changes of primary cultivated hippocampal neurons exposed to H 2 O 2 is involved in testosterone＇s neuronal protection. Methods： The primary cultivated hippocampal neurons exposed to H 2 O 2 were randomly devided into control group,H 2 O 2 group with different concentration,testosterone group. With Fluo- 3 labelled probe,the calcium levels were observed by Laser Confocal Scanning Microscopy（ LCSM） before and after tes- tosterone adding. Results： The calcium levels were significantly higher in 100 μM and 200 μM H 2 O 2 groups than in controls. Compared with 100 μM H 2 O 2 group,the intracellular calcium content in testosterone group with the same H 2 O 2 concentration was significantly decreased. Conclusion： In primary cultivated hippocampal neuron,free radical can increase the level of intracellu- lar calcium,and testosterone can attenuate the change.