针刺镇痛时家兔尾核头部不同区域阿片样肽和无机离子含量的变化

<正> 近年来的一些实验提示,尾核与痛觉及针刺镇痛作用有关。刺激尾核可以提高痛阈,可以加强电针效应,而损毁尾核可使电针效应有不同程度的减弱。对晚期癌肿引起恶痛用药物治疗效果不满意的病例,在尾核头部埋藏电极,刺激尾核,大部分病例可得到不同程度的镇痛效果。尾核内阿片样肽的含量很高,阿片样肽具有镇痛作用,这种作用可被纳洛酮部分阻断。无机离子在针刺镇痛中的作用,正逐渐被人们重视。根据目前的资料,尾核中的阿片样肽,主要是脑啡肽以及有关的六

THE RELATlONSHIP BETWEEN ACUPUNCTURE ANALGESIA AND OPIOID PEPTIDES OR INORGANIC ION IN RABBIT CAUDATE NUCLEUS

The quantities of opioid peptides, Cu~(2+) and Zn~(2+) were determined be- fore and after acupuncture in caudate nucleus perfusate. The cannula was sterotaxically implanted into caudate nucleus according to the atlas of Sawyer's (A_4, H_(4.5), L_3, or A_2, H_(3.5), L_3). The opioid peptides in the perfusate was purified through a column of Amberlite XAD-2 and deter- mined quantitatively by radioreceptorassay. Cu~(2+) and Zn~(2+) were determined by anodic stripping voltammetry. It was found that the quantities of opioid peptides in the anterior portion (A_4 or A_5) were increased significantly about 10.98±2.38% (N=10, P<0.002) after acupuncture and the increment was parallel to the rise of pain threshold; while in the posterior portion (A_1 or A_2) there was no change. After acupuncture for 30 minutes, the quantity of Zn~(2+)was increased significantly about 0.17±0.06 μg/ml (N=10, P<0.02) and the increment was parallel to the rise of pain threshold. The quantity of Cu~(2+) was decre- ased significantly about 0.37±0.12 μg/ml (N=10, p<0.01). This shows that the analgesic effect of acupuncture is related to opioid peptides, Cu~(2+) and Zn~(2+) in the anterior portion.