Coapresta2000全自动凝血分析仪检测CRP的可行性探索

目的探索Coapresta2000全自动凝血分析仪检测C反应蛋白(CRP)的可行性。方法测定Coapresta2000全自动凝血分析仪检测血浆CRP时的准确度、线性、精密度、携带污染率及两仪器(Ottoman全自动特定蛋白即时检测分析仪与Coapresta2000全自动凝血分析仪)的相关性。结果 Coapresta2000全自动凝血分析仪检测血浆CRP时的3个批号试剂平均回收率为106.0%,准确度良好;批内精密度小于10%,日间精密度小于15%;携带污染率0.32%;Ottoman全自动特定蛋白即时检测分析仪与Coapresta2000全自动凝血分析仪测定血浆CRP结果相关方程为Y=0.001 2 X+0.489,相关指数r2=0.997 5,差异无统计学意义(P0.01),两仪器测定血浆标本CRP结果高度相关;Coapresta2000全自动凝血分析仪检测血浆CRP时的线性为0~200mg/L。结论 Coapresta2000全自动凝血分析仪检测血浆CRP时批内精密度较好,携带污染率低,与Ottoman全自动特定蛋白即时检测分析仪高度相关,有进一步研究价值。

Study on feasibility of Coapresta2000 automatic coagulation analyzer for detecting CRP

Objective To explore the feasibility of the Coapresta2000 automatic coagulation analyzer for detecting C‐reactive protein(CRP) .Methods The performance of the Coapresta2000 automatic coagulation analyzer for detecting CRP was analyzed ,in‐cluding accuracy ,linearity ,precision ,carryover rate and the correlation between the Ottoman automatic special protein real time ana‐lyzer and the Coapresta2000 automatic coagulation analyzer .Results The men recovery rate of 3 batches of reagent by the Coapres‐ta2000 automatic coagulation analyzer for detecting CRP for detecting plasma CRP was 106 .0% with better accuracy ;the within‐run precision was less than 10% and the inter‐day precision was less than 15% ;the carryover rate was 0 .32% ;the related equation in the Ottoman automatic special protein real time analyzer and the Coapresta2000 automatic coagulation analyzer for detecting plas‐ma CRP was Y=0 .001 2X+0 .489 ,the correlation coefficient was r2 =0 .997 5 ,the difference was not statistically significant(P>0 .05) .The CRP results of plasma sample detected by these two instruments was highly correlative ;the linearity in the Coapres‐ta2000 automatic coagulation analyzer for detecting CRP was 0-200 mg/L .Conclusion The Coapresta2000 automatic coagulation analyzer for detecting CRP has better within‐run precision ,low carryover rate ,high correlation with the Ottoman automatic special protein real time analyze ,which has a value for further study .