湖北省首例输入性卵形疟原虫wallikeri亚种的病原学诊断分析

目的应用巢式PCR技术对1例罕见卵形疟原虫wallikeri亚种进行诊断和鉴定。方法采集初诊为输入性间日疟患者血样,进行疟疾快速诊断试剂盒、显微镜镜检和巢式PCR检测,并进行测序分析。结果该患者疟疾快速诊断试剂盒检测阴性;镜检查见寄生于红细胞内的疟原虫环状体和滋养体;采用卵形疟原虫wallikeri亚种特异性引物(rOVA1v/rOVA2v)进行巢式PCR,在760bp处有特异性扩增条带,测序后经Blast比对,与GenBank数据库中卵形疟原虫wallikeri亚种部分序列的一致性为99%。结论该患者经巢式PCR和序列分析确诊为湖北省首例卵形疟原虫wallikeri亚种感染。

Analysis on etiological diagnosis of first case of imported Plasmodium ovale wallikeri subspecies in Hubei Province

Objective To use the nested PCR technology to diagnose and identify a case of imported Plasmodium ovale wallikeri subspecies .Methods Blood sample was collected from 1 case of initially diagnosed imported tertian malaria and performed the ex‐amination of microscopy ,rapid diagnostic tests (RDTs) and nested‐PCR .Moreover the sequencing was conduced .Results RDTs showed the negative result ;the ring form and trophozoite of Plasmodium could be observed in the blood smear by microscopy ;the Plasmodium ovale wallikeri subspecies specific primer rOVA 1v/rOVA2v was adopted for conducting nested PCR ,the specicific am‐plification band appeared at 760 bp ,after sequencing and Blast aligning ,its coincidence with the partial sequence of Plasmodium ovale wallikeri subspecies in the Genbank database was 99% .Conclusion This patient is the first case of Plasmodium ovale wallik‐eri subspecies infection in Hubei province by nested PCR and sequencing analysis .