| CD4~+CD25~+调节性T细胞对内皮细胞抗原提呈功能的影响及机制 |
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| 引用本文： | 何少林,李大主,黎明,马旭明,林静,昌薇,赵卉.CD4~+CD25~+调节性T细胞对内皮细胞抗原提呈功能的影响及机制[J].中国免疫学杂志,2011,27(5):391-394,403. |
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| 作者姓名： | 何少林 李大主 黎明 马旭明 林静 昌薇 赵卉 |
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| 作者单位： | 华中科技大学同济医学院协和医院心内科,武汉,430022 |
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| 摘 要： | 目的:探讨CD4+CD25+调节性T细胞(Treg)对内皮细胞抗原提呈功能的影响及机制。方法:磁性细胞分离器(MACS)分离CD4+CD25+T细胞及CD4+CD25-T细胞。在ox-LDL作用下,HUVECs与CD4+CD25+T细胞共培养,24小时后收集HUVECs。应用流式细胞术测定HUVECs抗原提呈分子(HLA DR,CD86,CD80)的表达,Cell Counting Kit-8法(CCK-8)测定HU-VECs刺激CD4+CD25-T细胞增殖的能力,Transwell小室实验初步探讨Treg作用于HUVECs的具体机制。结果:与对照组比较,Treg可显著抑制HUVECs抗原提呈分子的表达及刺激T细胞增殖的能力。用Transwell隔离后,与or-LDL刺激组比较,HU-VECs抗原提呈分子表达及其刺激T细胞增殖的能力无明显变化。结论:Treg可显著抑制HUVECs抗原提呈能力,其作用机制可能为下调CD86的表达,且依赖细胞直接接触。
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| 关 键 词： | CD4+CD25+调节性T细胞 人脐静脉内皮细胞系 氧化型低密度脂蛋白 抗原提呈分子 细胞增殖 Transwell |
CD4+CD25+ regulatory T cells modulate antigen-presenting function of human umbilical vein endothelial cells |
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| HE Shao-Lin,LI Da-Zhu,LI Ming,MA Xu-Ming,LIN Jing,CHANG Wei,ZHAO Hui.CD4+CD25+ regulatory T cells modulate antigen-presenting function of human umbilical vein endothelial cells[J].Chinese Journal of Immunology,2011,27(5):391-394,403. |
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| Authors: | HE Shao-Lin LI Da-Zhu LI Ming MA Xu-Ming LIN Jing CHANG Wei ZHAO Hui |
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| Institution: | HE Shao-Lin,LI Da-Zhu,LI Ming,MA Xu-Ming,LIN Jing,CHANG Wei,ZHAO Hui.Institute of Cardiology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China |
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| Abstract: | Objective:To investigate whether and how Treg affect the antigen-presenting function of HUVECs,and the mechanism of Treg in the development of atherosclerosis.Methods:Treg were isolated from human peripheral blood mononuclear cells(PBMCs) which obtained from normal volunteers by magnetic cell sorting-column and analyzed by Flow Cytometry.HUVECs were incubated alone(control) or with anti-CD3 mAbs activated Treg(CD25+) and ox-LDL.After 24 h,HUVECs were collected and the expression of HLA DR,CD80 and CD86 were... |
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| Keywords: | Transwell |
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