|
|||||
|
|
| HPLC法测定银黄片中绿原酸与黄芩苷含量 | |
| 引用本文： | 袁浩,刘永利,李冬梅. HPLC法测定银黄片中绿原酸与黄芩苷含量[J]. 中国民族民间医药杂志, 2011, 20(1): 61-62 |
| 作者姓名： | 袁浩 刘永利 李冬梅 |
| 作者单位： | 河北省药品检验所,河北,石家庄,050011 |
| 摘 要： | 目的：建立银黄片中绿原酸与黄芩苷的含量测定方法。方法：C18柱（4．6×150mm，5μm，shim-pack，Shimadzu），流动相：乙腈与0．1％磷酸溶液进行梯度洗脱，检测波长为318nm，流速为1ml·min-1，柱温为室温。结果：绿原酸加样回收率为100．22％，RSD为1．49％；黄芩苷加样回收率为100．45％，RSD为1．16％。结论：方法简便、准确，可用于银黄片的质量控制。 |
| 关 键 词： | 银黄片 梯度洗脱 绿原酸 黄芩苷 高效液相色谱法 |
Determination of chlorogenic acid and baical in Yinhuang Tablets by HPLC |
|
| YUAN Hao,LIU Yong-li,LI Dong-mei. Determination of chlorogenic acid and baical in Yinhuang Tablets by HPLC[J]. Chinese Journal of Ethnomedicine and Ethnopharmacy, 2011, 20(1): 61-62 | |
| Authors: | YUAN Hao LIU Yong-li LI Dong-mei |
| Affiliation: | YUAN Hao, LIU Yong- li, LI Dong- mei ( Hebei Provincial Institute for Drug Control, Shijiazhuang 050011 ) |
| Abstract: | Objective： To establish a HPLC method for determination of chlorogenic acid and baicalin in Yinhuang tablets. Method： In this method, the Cls column （4. 6 ×150mm,5μmm, shim- pack, Shimadzu） was used as the stationary phase, acetonitrile -0. 1% phosphoric acid as gradient eluent, the flow rate was 1.0ml·min-1 and detection wavelength was at 318nm, the tempera- ture of column was the room temperature. Result： The chlorogenic acid recovery of the added sample was 100. 22% and RSD was 1.49%. The baicalin recovery of the added sample was 100. 45% and RSD was 1.16%. Conclusion： This methods was simple, accurate and can be used for the quality control of YinHuang Tablets. |
| Keywords: | YinHuang Tablets Gradient elution chlorogenic acid baicalin HPLC |
| 本文献已被 维普 万方数据 等数据库收录！ | |