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| 余甘子提取物体外抗单纯疱疹病毒1型和2型的初步研究 | |
| 引用本文： | 瞿畅,赖志才,裴赢,张美英,王一飞,张颖君,杨崇仁. 余甘子提取物体外抗单纯疱疹病毒1型和2型的初步研究[J]. 时珍国医国药, 2010, 21(4) |
| 作者姓名： | 瞿畅 赖志才 裴赢 张美英 王一飞 张颖君 杨崇仁 |
| 作者单位： | 1. 暨南大学药学院,广东,广州,510632;暨南大学生物医药研究开发基地,广东,广州,510632;基因工程药物国家工程研究中心,广东,广州,510632 2. 暨南大学生物医药研究开发基地,广东,广州,510632;基因工程药物国家工程研究中心,广东,广州,510632 3. 中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,云南,昆明,650204 |
| 基金项目： | 国家自然科学基金，植物化学与西部植物资源持续利用国家重点实验室自主课题，开放课题 |
| 摘 要： | 目的 从余甘子的醋酸乙酯提取物、甲醇提取物和水提物3种提取物体外筛选抗单纯疱疹病毒1型和2型的活性成分.方法 通过观察药物毒性、病毒引起的细胞病变效应、空斑减数法,判断药物的毒性及其抗病毒效应.结果 余甘子的醋酸乙酯提取物、甲醇提取物和水提物对于单纯疱疹病毒1型的半数抑制浓度分别为25.28,66.17,100.94 μg/ml;余甘子的3种提取物对于单纯疱疹病毒2型的半数抑制浓度分别为31.70,180.3,112.1 μg/ml.结论 余甘子3种提取物在体外对单纯疱疹病毒1型和2型均有一定的抑制作用. |
| 关 键 词： | 余甘子 单纯疱疹病毒1型 单纯疱疹病毒2型 细胞病变效应法 空斑减数法 |
Study on the Anti-HSV Activity of Crude Extract from Phyllanthus emblica in vitro |
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| QU Chang,LAI Zhi-cai,PEI Ying,ZHANG Mei-ying,WANG Yi-fei,ZHANG Ying-jun,YANG Chong-ren. Study on the Anti-HSV Activity of Crude Extract from Phyllanthus emblica in vitro[J]. Lishizhen Medicine and Materia Medica Research, 2010, 21(4) | |
| Authors: | QU Chang LAI Zhi-cai PEI Ying ZHANG Mei-ying WANG Yi-fei ZHANG Ying-jun YANG Chong-ren |
| Affiliation: | QU Chang1,2,3,LAI Zhi-cai1,PEI Ying1,ZHANG Mei-ying2,WANG Yi-fei2,ZHANG Ying-jun4,YANG Chong-ren4 (1.College of Pharmacology,Jinan University,Guangzhou 510632,China,2.Biomedicine Research , Development Center of Jinan University,3.National Engineering Research Center of Genetic Medicine,4.State Key Laboratory of Phytochemistry , Plant Resources in West China,Kunming Institute Botany,Chinese Academy of Sciences,Kunming 650204,China) |
| Abstract: | Objective To study the active components of anti- HSV-1 and HSV-2 of crude extract from Phyllanthus emblica.Methods The toxic and antivirous effects were evaluated by observing toxic effect,cytopathic effect(CPE),MTT colorimetric assay for vital cell rate,plaques reduction ratio.Results Inhibition on herpes simplex virus were observed during the experiment.The IC_(50) on HSV-1 of acetic ether extract,methanol extract,aqueous extract from Phyllanthus emblica were 25.28,66.17,100.94μg/ml; while the IC_(50) on HSV-2 of the extracts above were 31.70,180.3,112.1μg/ml.Conclusion The three extracts from Phyllanthus emblica can inhibit both of HSV-1 and HSV-2 in vitro. |
| Keywords: | Phyllanthus emblica HSV-1 HSV-2 CPE Plaques reduction ratio |
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