复方甘芍贴剂对多囊卵巢综合征大鼠模型性激素及肝脏转氨酶的影响

目的探讨复方甘芍贴剂对多囊卵巢综合征(polycistic ovarian syndrome,PCOS)模型大鼠性激素及肝脏转氨酶水平的影响。方法应用Kafali造模法建立PCOS动物模型。将大鼠随机分为正常对照组、P-COS模型组(模型组)、达英-35组、复方甘芍胶囊组及复方甘芍贴剂组,灌肠及贴药21天后应用放免法测定大鼠性激素水平,酶偶联速率法检测肝脏转氨酶水平。结果与正常对照组比较,模型组大鼠血清黄体生成素(luteinizing hormone,LH)、睾酮(testosterone,T)及卵泡刺激素(follicle stimulating hormone,FSH)水平升高,血清雌二醇(estradiol,E2)、孕酮(progesterone,P)水平下降,差异有统计学意义(P<0.05)。与模型组比较,3个用药组大鼠血清LH、T及FSH水平下降,血清E2、P水平升高,差异有统计学意义(P<0.05)。正常对照组、模型组和复方甘芍贴剂组大鼠血清谷丙转氨酶(alanine aminotransferase,ALT)及谷草转氨酶(aspartate aminotransferase,AST)水平比较差异无统计学意义(P>0.05);复方甘芍贴剂组ALT及AST低于达英-35组及复方甘芍胶囊组,差异有统计学意义(P<0.05)。结论复方甘芍贴剂通过调整PCOS模型大鼠内分泌功能,从而改善卵巢功能,促进卵泡发育及排卵;该贴剂不增加大鼠肝脏负担,体现了贴剂的作用优势。

Effects of Compound Ganshao Paste on the Levels of Sex Hormones and Hepatic Transaminases in Polycystic Ovarian Syndrome Rat Model

Objective To study the effects of Compound Ganshao Paste(CGP) on the levels of sex hormones and hepatic transaminases in polycystic ovarian syndrome(PCOS) rat model.Methods The PCOS rat model was established using Kafali modeling method.Rats were randomly divided into five groups,i.e.,the normal control group,the PCOS model group,the Diane-35 group,the Compound Ganshao Caspule group,and the CGP group.The levels of sex hormones were detected using radioimmunoassay.The levels of hepatic transferases were detected using method of enzyme coupling ratio.Results Compared with the normal control group,the serum levels of luteinizing hormone(LH) ,testosterone(T) ,follicle stimulating hormone(FSH) ,estradiol(E2) ,and progesterone(P) decreased,showing statistical difference(P<0.05) .Compared with the model group,the serum levels of LH,T,and FSH decreased,while serum E2 and P increased in the three medication groups,showing statistical difference(P<0.05) .There was insignificant difference in the serum levels of alanine aminotransferase(ALT) and aspartate aminotransferase(AST) among the normal control group,the model group,and the CGP group(P>0.05) .Compared with the Diane-35 group and the Compound Ganshao Capsule group,the levels of hepatic transaminases of the CGP group was lower with statistical difference(P<0.05) .Conclusions CGP could improve the ovarian functions through adjusting the endocrine functions of PCOS model rats,thus stimulating the follicular development and ovulation.CGP did not add the hepatic burden.