地榆鞣质抗肝癌细胞SMMC—7721的MTT及FCM分析

探讨中药成分地榆鞣质的抗癌活性，并试图建立一种筛选抗癌药物的方法，方法：运用ＭＴＴ法测定ＳＴＭ及ＳＴＬ对体外培养人肝癌ＳＭＭＣ－７７２１细胞的杀伤率并通过流式细胞仪分析细胞分裂周期各时象ＤＮＡ变化。结果；ＳＴＭ与ＳＴＬ均有明显抗癌活性，与阴性对照组相比有显著差异，并具有剂量效应关系，ＳＴＭ，ＳＴＬ与ＭＭＣ联合用药抗癌活性增强，与单独用药组相比差异显著；

Antitumor-effect of Sanguisorba officinalis tannins on liver cancer cell SMMC-7721 by MTT and FCM

Aim: To investigate the antitumor effect of Chinese Herb Sanguisorba officinalis tannins (ST) and to find amethod of screening antitumor drugs. Methods: Human liver cancer cell SMMC-7721 cells were cultured in vitro andthen treated with each kind of drug. Tumor cell killing ratewas analyzed by MTT method and DNA content alterationof each phase of cell circle was determined by FCM (flowcytometry)assay. Results: STM and STL both had strong activity of antitumor does~dependent effect (RM =0. 9615, RL= 0. 9531 ). Compared with negative control group, both ofthem had significant difference (P<0.01). Combined withMMC, their antitumor acitivity was stronger than singledrug group (P<0. 01 ). The flow cytometry assay of cell-circle DNA content displayed that S-phase cell DNA contentdecreased significantly. Prolifelation index (PI)delined andapoptosis peak (AP peak)was introduced. Conclusion: FCMprovides a new way of screening the antitumor component ofChinese herb.