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| 人重组可溶性肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导急性髓系白血病细胞凋亡的实验研究 | |
| 引用本文： | 王红祥,邹萍,赵湜. 人重组可溶性肿瘤坏死因子相关凋亡诱导配体(TRAIL)诱导急性髓系白血病细胞凋亡的实验研究[J]. 中国实验血液学杂志, 2004, 12(6): 766-769 |
| 作者姓名： | 王红祥 邹萍 赵湜 |
| 作者单位： | 华中科技大学同济医学院附属协和医院血液科,武汉,430022 |
| 摘 要： | 为了研究肿瘤坏死因子相关凋亡诱导配体(TRAIL)对急性髓系白血病(AML)细胞的作用，通过MTT比色法测定不同浓度TRAIL对39例AML患者(病例组)和21例健康人(对照组)的骨髓或外周血单个核细胞的杀伤率。用流式细胞术检测细胞凋亡。结果发现，不同浓度TRAIL对AML细胞均有不同程度的杀伤作用，而对正常细胞无此作用。结论：TRAIL能通过诱导细胞凋亡而杀伤AML细胞，是一种有望应用于临床白血病治疗的新型生物制剂。 |
| 关 键 词： | 肿瘤坏死因子 相关凋亡诱导配体 急性髓系白血病 急性髓系白血病细胞 细胞凋亡 TRAIL |
| 文章编号： | 1009-2137(2004)06-0766-04 |
| 修稿时间： | 2003-12-18 |
Apoptosis of Acute Myeloid Leukemic Cells Induced by Human Recombinant Soluble Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL) |
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| WANG Hong Xiang,ZOU Ping,ZHAO Shi. Apoptosis of Acute Myeloid Leukemic Cells Induced by Human Recombinant Soluble Tumor Necrosis Factor-Related Apoptosis Inducing Ligand (TRAIL)[J]. Journal of experimental hematology, 2004, 12(6): 766-769 | |
| Authors: | WANG Hong Xiang ZOU Ping ZHAO Shi |
| Affiliation: | Department of Hematology, The Affiliated Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China. ljwhx@21cn.com |
| Abstract: | In order to investigate the effect of tumor necrosis factor related apoptosis inducing ligand(TRAIL) on acute myeloid leukemic(AML) cells, BMMNC or PBMNC of 39 cases of AML (patient group) and 21 normal persons(control group) were isolated; the cytotoxic effects of TRAIL at various concentration on the cells were evaluated by MMT, apoptosis were detected by flow cytometry. The results showed that TRAIL of various concentration could kill AML cells in various degrees, but had no killing effect on normal cells. It is concluded that TRAIL can kill AML cells through inducing apoptosis, and TRAIL has a promising prospect in clinical use. |
| Keywords: | tumor necrosis factor related apoptosis inducing ligand acute myeloid leukemia AML cell apoptosis TRAIL |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |