恙虫病立克次体组织培养抗原在血清学调查的应用

本文试验表明恙虫病立克次体用组织培养法所制备的抗原与其它微生物免疫血清作补体结合试验，其交叉滴度未超过1：4，故≥1：8的血清滴度可视为特异性反应，85份发热病人及恙虫病既往患者血清用不同来源菌株制备的混合抗原作补体结合试验，同时用外斐氏试验作比较，阳性率分别为72.9%及48.2%，差别十分显著（χ²=9.8， P<0.01）。另外，644份流行区及56份非流行区正常人血清，补体结合试验阳性率分别为38.6%及3.5%。说明本抗原具有较高的特异性和敏感性，可用于本病的临床实验室诊断和血清学调查。

Application of Rickettsia tsutsugamughi Tissue Culture Antigen in Serological Investigation

The titer of the antisera obtained by immunization with microbes other than R.tsutsugamushi was 1:4 or lower in CFT with R.tsutsugamushi tissue culture antigen. A serum giving 1:8 or higher reactivity with the antigen was considered as a specific reaction. Eighty five specimens of serum obtained from febrile patients and former patients with Tsutsugamushi disease were examined simultanously by both CFT and Weil-Felix test. It was shown that the positive rate in CFT was 72.9% and in Weil-Felix test 48.2%. The difference between them was significant (χ²=9.8, P<0.01). In addition, the positive rate of CFT in 644 specimens of serum obtained from population in endemic area was 38.6% while that in 56 specimens in non-endemic area was 3.5%. The results showed that the antigen was quite specific and sensitive for detecting the antibody against R.tsutsugamushi.