雷公藤多甙对小鼠生育的影响及肉苁蓉干预作用的研究

目的:研究使用不同时间和剂量的雷公藤多甙(GTW)对昆明小鼠怀孕率的影响及肉苁蓉干预和停用GTW后的自愈倾向。方法:将90只成年雄性昆明鼠随机分为9组(每组10只)。对照组给予1%羧甲基纤维素(CMC)溶液;GTW各组分别给予45.0、30.0、22.5、15.0、7.5mg/(kg·d)GTW混悬液;肉苁蓉各组分别给予30.0、7.5mg/(kg·d)GTW混悬液;停药组给予30.0mg/(kg·d)GTW混悬液。以上各组先灌胃20d,20d后与成年雌鼠1∶2合笼7d。第28d起GTW各组按原剂量继续给药;肉苁蓉各组给原剂量GTW混悬液的同时分别给10.0、2.5g/(kg·d)肉苁蓉煎液;停药组停GTW,改为1%CMC溶液。20d后再次合笼7d。每次合笼后观察雌鼠怀孕率。结果:随着GTW给药时间的延长和剂量的增加,雌鼠怀孕率呈下降趋势。GTW45.0mg/(kg·d)组给药40d后怀孕率降为0%(P<0.01)。肉苁蓉10.0g/(kg·d)组怀孕率由20d时的25%上升到40d时的55%。停药组怀孕率由25%上升到56%,与对照组比较,差异无显著性(P>0.05)。结论:GTW对雄性小鼠的生殖毒性呈时间和剂量依赖性。肉苁蓉可以改善GTW对雄鼠生殖系统的抑制作用,提高雌鼠的怀孕率。停用GTW20d,怀孕率可以基本恢复正常。

Effects of Glycosides of Tripterygium Wilfordii and Cistanche Deserticola on the Fertility of Male Mice

Objective ： To study the impact of glycosides of tripterygium wilfordii （GTW） on fertility, and the reversibility of this im- pact following intervention with cistanche deserticola （CD） and after withdrawal of GTW. Methods ： Ninety adult male KM mice were equally and randomly divided into 9 groups： 5 GTW groups, treated with GTW at 45.0, 30.0, 22.5, 15 and 7.5 mg/kg · d, respectively, ：2 CD groups, administered GTW at 30.0 and 7.5 mg/kg · d, respectively, a drug withdrawal group, given GTW at 30.0 mg/ kg · d, and a control group, given 1% CMC solution, all intragastrically for 20 days. Then they were mated with female mice at the ratio of 1 ： 2 for 7 days, followed by another 20 days of medication, the GTW groups at the same doses, the CD groups given CD additionally at 10.0 g/kg · d and 2.5 g/kg · d respectively, and the withdrawal group given 1% CMC solution only. Again they were mated with the same females, and the pregnancy rate was observed after each mating. Results： The pregnancy rate dropped with the increase of the dose and medication time of GTW, which was 0% at 40 d in the 45.0 mg/kg · d GTW group （ P 〈 0.01 ）, but rose from 25% at 20 d to 55% at 40 d in the 10.0 g/kg · d CD group, and from 25% at 20 d to 56 % at 40 d in the withdrawal group. Conclusion： The reproductive toxicity of GTW acts on male mice in a time- and dose-dependent manner, which can be reduced by Cistanche deserticola. The fertility of the male mice can be restored to normal 20 days after the cessation of GTW.