LDN-193189对人去分化软骨肉瘤细胞系NDCS-1 的抑制作用研究* |
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引用本文: | 杨康,汤小东,郭卫.LDN-193189对人去分化软骨肉瘤细胞系NDCS-1 的抑制作用研究*[J].中国肿瘤临床,2016,43(2):52-55. |
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作者姓名: | 杨康 汤小东 郭卫 |
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作者单位: | 作者单位:北京大学人民医院骨肿瘤科(北京市100044) |
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基金项目: | 国家自然科学基金,教育部新世纪人才项目 |
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摘 要: | 目的:检测骨形成蛋白(BMP )受体抑制剂LDN-193189对人去分化软骨肉瘤(DDCS)细胞系NDCS- 1 的抑制作用,探讨LDN-193189对去分化软骨肉瘤的抑癌机制。方法:以5 nmol/L 的LDN-193189作用于NDCS- 1 细胞,MTT 、平板克隆法检测LDN-193189对NDCS- 1 细胞的增殖抑制作用,Transwell 法、划痕实验检测LDN193189对NDCS- 1 细胞的侵袭抑制作用,Westernblot检测BMPR 2、p-Smad1/ 5 及RUNX 2 的蛋白表达抑制情况。结果:药物处理后NDCS- 1 细胞增殖、侵袭被明显抑制;药物处理后NDCS- 1 细胞的BMPR 2、p-Smad1/ 5 及RUNX 2 蛋白表达下降。结论:LDN-193189通过抑制BMPR 2-p-Smad 1/ 5-RUNX2 信号传导通路能有效抑制去分化软骨肉瘤细胞系NDCS- 1 的增殖侵袭能力。
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关 键 词: | 去分化软骨肉瘤 LDN-193189? NDCS- 1? BMPR 2? p-Smad1/ 5? RUNX 2 |
收稿时间: | 2015-11-17 |
LDN-193189 inhibits progression and induces apoptosis in human dedifferentiated chondrosarcoma cell line NDCS-1 |
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Institution: | Department of Bone Oncology, Peking University, People's Hospital, Beijing100044, China |
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Abstract: | Objective:To clarify the effects of the BMP receptor inhibitor LDN-193189 in the dedifferentiated chondrosarcoma (DDCS) cell line NDCS-1 and to explore the anti-tumor mechanism of LDN-193189 in DDCS. Methods:NDCS-1 was treated with 5 nmol/L of LDN-193189. MTT assay and clone formation experiments were used to verify that LDN-193189 suppressed cel proliferation. Transwel and wound healing tests were performed to demonstrate that LDN-193189 inhibited cell invasion. Western blot detection was used to show that LDN-193189 inhibited the suppression of BMPR2, p-Smad1/5, and RUNX2 protein expression. Results:The BMPR2 signaling pathway was inhibited by LDN-193189;thus, cell viability and invasion were significantly suppressed. Conclusion:LDN-193189 induces the inhibition of progression in vitro via the BMPR2-p-Smad1/5-RUNX2 signaling pathway in the human DDCS cell line NDCS-1. |
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Keywords: | dedifferentiated chondrosarcoma LDN-193189 NDCS-1 BMPR2 p-Smad1/5 RUNX2 |
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