紫茎泽兰提取物对小鼠脾细胞凋亡的影响

目的 观察紫茎泽兰提取物对小鼠脾细胞凋亡的影响。方法 小鼠分别以0、75、150、300 mg/kg的剂量经口给予紫茎泽兰提取物,连续7 d后,无菌条件下取小鼠脾脏,制备脾细胞悬液,在RPMI 1640培养基中培养24~48 h,流式细胞术观察脾细胞凋亡发生率,光镜下观察脾脏组织病理学变化。结果 紫茎泽兰提取物各剂量组脾细胞早期凋亡率、晚期凋亡率及死亡率都明显增加。脾脏组织病理学检查可见,红白髓界限不清,白髓减少或消失,淋巴小结结构破坏,淋巴细胞数量较少,排列疏松,皮质层变薄。结论 紫茎泽兰提取物可引起脾脏组织病理学明显改变,脾细胞凋亡率明显增加。

Effects of Eupatorium Adenophorum Spreng Extract on Apoptosis of Splenocytes in Mice

Objective To observe apoptosis of splenocytes in mice with Eupatorium adenophorum spreng extract. Methods The mice were gavaged Eupatorium adenophorum spreng extract at dose of 75. 0、150. 0、300. 0 g·kg1·d-1 for 7 days. The spleens were excised from mice under aseptic condition,and single-cell suspension of splenocytes was prepared. After incubation in RPMI 1640 medium supplemented for 24 ~ 48 hours,apoptotic rate of splenocytes was measured. The pathological changes in spleen were observed by optical microscopy. Results By optical microscopy,white pulps were atrophied and splenic nodules were destroyed,cell dissolved fragments. A significant increase of a apoptotic rate in Eupatorium adenophorum spreng extract treate groups compared with the control Conclusion Eupatorium adenophorum spreng extract could cause obvious pathological changes and significant increase of a apoptotic rate in the mouse spleen.