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| 磷酸镁骨粘合剂的Ames致突变试验研究 | |
| 引用本文： | 张秉文,俞永林,刘昌胜,郭瀚,屈卫东,蒋颂辉,黄煌渊. 磷酸镁骨粘合剂的Ames致突变试验研究[J]. 中国骨与关节损伤杂志, 2009, 24(10) |
| 作者姓名： | 张秉文 俞永林 刘昌胜 郭瀚 屈卫东 蒋颂辉 黄煌渊 |
| 作者单位： | 1. 复旦大学附属华山医院骨科,上海市,200040 2. 华东理工大学生物材料研究所 3. 复旦大学公共卫生学院卫生学教研室 |
| 基金项目： | 上海市科学技术委员会基础处重点项目(07JC14008) |
| 摘 要： | 目的 从基因角度探讨磷酸镁骨粘合剂(MPC)的遗传毒理学特性,为MPC的临床应用进一步提供生物安全性依据.方法 将磷酸镁骨粘合剂制备浸提液,采用Ames致突试验,以生理盐水为阴性对照,测试其对各种鼠伤寒沙门菌(TA100、TA102、TA97和TA98)的致突变比值(MR).MR=实验组回变菌落数(Rt)/阴性对照组回变菌落数(Rc).结果 阳性对照组各菌株回变菌落平均数均超过相应阴性对照组回变菌落平均数的2倍以上(MR＞2),表明此浸提液各剂量组对鼠伤寒沙门菌的致突变比值MR均小2.结论 MPC不会引起鼠伤寒沙门菌的回复突变数增加,提示其不会引起基因突变. |
| 关 键 词： | 磷酸镁 骨粘合剂 Ames致突变试验 |
Ames Test of Magnesium Phosphate Cement |
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| Zhang Bingwen,Yu Yonglin,Liu Changsheng,et al.. Ames Test of Magnesium Phosphate Cement[J]. Chinese Journal of Bone and Joint Injury, 2009, 24(10) | |
| Authors: | Zhang Bingwen Yu Yonglin Liu Changsheng et al. |
| Affiliation: | Zhang Bingwen,Yu Yonglin,Liu Changsheng,et al.Department of Orthopaedics,Huashan Hospital,Fudan University,Shanghai,200040 |
| Abstract: | Objective To study the inherited toxicology of magnesium phosphate cement(MPC) from the aspects of DNA in order to study the biological safety of the MPC for clinical use in the future.Methods The diffusion of magnesium phosphate cement and different kind of murine typhoid salmonella(TA100,TA102,TA97 and TA98 ) were compounded respectively.Then these compounds were used in the Ames test and the nature saline was used as the control group.Then the mutagenesis rates(MR) of murine typhoid salmonella were calcu... |
| Keywords: | Magnesium phosphate cement Adhesive cement of bone Ames mutagenic test |
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