甲磺酸去铁胺抑制精索静脉曲张睾丸支持细胞模型铁死亡的研究

目的:探究甲磺酸去铁胺对精索静脉曲张睾丸支持细胞氧化应激模型的保护作用。方法:细胞实验选择小鼠睾丸支持细胞TM-4，设置对照组(A组)、甲磺酸去铁胺+对照组(B组)、氯化钴组(C组)、甲磺酸去铁胺+氯化钴组(D组)。C组和D组加入400 μmol/L氯化钴，随后培养细胞24 h构建精索静脉曲张氧化应激模型。B组和D组加...

Deferoxamine mesylate inhibits ferroptosis in testicular Sertoli cell model of varicocele

Objective To explore the protective effect of deferoxamine mesylate on oxidative stress model of testicular Sertoli cells with varicocele.Methods In the cell experiment,mouse testicular Sertoli cells TM-4 were selected,and control group(group A),deferoxamine mesylate+control group(group B),cobalt chloride group(group C),and deferoxamine mesylate+cobalt chloride group(group D)were set up.In groups C and D,400μmol/L cobalt chloride was added and the cells were cultured for 24 h to construct the oxidative stress model of varicocele.In groups B and D,800μmol/L deferoxamine mesylate was added.Cell counting kit-8(CCK-8)assay and flow cytometry were used to determine the survival and apoptosis of cells.The levels of reactive oxygen species(ROS),sodium dodecyl sulfate(SDS),malondialdehyde(MDA),and glutathione(GSH)-PX in cells were measured by enzyme linked immunosorbent assay(ELISA).Western blotting was used to detect the protein expression level of glutathione peroxidase-4(GPX4).The t-test was used for comparison between groups.Results The CCK-8 assay showed that the survival rate in group C and group D was lower than in group A[(45.75±12.08)%,(86.53±2.57)%vs.(100.00±0.00)%,t=11.000,12.810,P<0.05],and the difference was statistically significant,and the survival rate in group D was higher than that in group C[(86.53±2.57)%vs.(45.75±12.08)%,t=8.087,P<0.01].Flow cytometry showed that the proportion of apoptotic cells in group D was lower than that in group C[(8.63±0.11)%vs.(34.19±0.35)%,t=121.300,P<0.01].The results of ELISA showed that the relative expression levels of ROS and MDA in group D were lower than those in group C[(239.17±23.18)%,(40.34±1.50)nmol/(L·mg)ratio(378.00±24.84)%,(49.18±2.68)nmol/(L·mg),t=10.010,7.053,P<0.01].The expression levels of superoxide dismutase(SOD)and glutathione peroxidasc(GSH-Px)in group D were higher than those in group C[(7.85±0.24)U/mg,(425.60±12.21)U/mg ratio(4.69±0.28)U/mg,(208.96±7.00)U/mg,t=20.650,37.710,P<0.01],and the difference was statistically significant.Western blotting showed that cobalt chloride hypoxia induced the down-regulation of the expression of iron death key protein GPx4,while deferoxamine mesylate restored the expression of iron death key protein GPX4.Conclusion Deferoxamine mesylate has a protective effect on the oxidative stress model of testicular Sertoli cells in varicocele by inhibiting ferroptosis.