| 抗人精子甘露糖受体抗血清对精子穿透实验的影响 |
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| 引用本文： | 江澍,林典梁,江一平.抗人精子甘露糖受体抗血清对精子穿透实验的影响[J].解剖学报,2007,38(6):727-730. |
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| 作者姓名： | 江澍 林典梁 江一平 |
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| 作者单位： | 1福建医科大学细胞与发育工程研究中心， 福州 350004； 2福建中医学院中西医结合系， 福州 350108 |
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| 基金项目： | 国家自然科学基金
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福建省自然科学基金
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福建省教育厅科研项目 |
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| 摘 要： | 目的 研究人精子甘露糖受体(MR)在精卵融合中的作用.方法 用亲合层析法分离纯化的人精子甘露糖受体来免疫Balb/c小鼠得到抗MR抗血清;用抗血清处理顶体反应后的人精子,并进行去透明带金黄地鼠卵的精子穿透实验(SPA),观察其体外受精情况.结果 抗MR抗血清可抑制人精子与去透明带金黄地鼠卵的结合与穿透,使卵子的受精率下降,具剂量依赖性.结论 人精子MR与精卵膜的识别与融合有关.
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| 关 键 词： | 甘露糖受体 人精子 精卵融合 受精 金黄地鼠卵 |
| 收稿时间： | 2007-02-27 |
| 修稿时间： | 2007-05-04 |
EFFECTS ON SPERM OOCYTE PENETRATING BY ANTI SERUM TO HUMAN SPERM MANNOSE RECEPTOR |
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| JIANG Shu,LIN Dian-liang,JIANG Yi-ping.EFFECTS ON SPERM OOCYTE PENETRATING BY ANTI SERUM TO HUMAN SPERM MANNOSE RECEPTOR[J].Acta Anatomica Sinica,2007,38(6):727-730. |
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| Authors: | JIANG Shu LIN Dian-liang JIANG Yi-ping |
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| Institution: | 1 Center of Cell Developmental Biology,Fujian Medical University,Fuzhou 350004,China；2 Department of Integrative Medicine, Fujian College of Traditional Chinese Medicine, Fuzhou 350108,China) |
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| Abstract: | Objective To study the effect of mannose receptor(MR) from human spermatozoa on sperm-egg fusion. Methods The Balb/c mouse was immunized with the purified mannose receptor(pMR) which was isolated from motile human sperm by mannose-agarose gel affinity chromatography and the anti-MR serum was harvested. Human sperm after induction of acrosome reaction was pretreated with anti-MR serum and subsequently processed in sperm penetration assay (SPA). Results The anti-MR serum can inhibit human spermatozoa from binding and penetrating zona-free golden hamster eggs with a dose-dependent reduction of Fertilization rate(FR) and Penetration index(PI).Conclusion The results indicate that MR of human sperm plays an important role in sperm-oocyte fusion. |
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| Keywords: | Mannose receptor Human sperm Sperm-egg fusion Fertilization Golden hamster egg |
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