| TaqMan实时荧光定量PCR检测肺炎链球菌 |
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| 引用本文： | 颜善活,孙雷,符可鹏,龙驹,叶海明,劳可干,樊祖茜,黄永霞. TaqMan实时荧光定量PCR检测肺炎链球菌[J]. 国外医学:临床生物化学与检验学分册, 2014, 0(2): 140-141 |
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| 作者姓名： | 颜善活 孙雷 符可鹏 龙驹 叶海明 劳可干 樊祖茜 黄永霞 |
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| 作者单位： | 钦州市妇幼保健院,广西钦州535000 |
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| 基金项目： | 2010年钦州市科学研究与技术开发计划项目（20100913）. |
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| 摘 要： | 目的利用实时荧光定量聚合酶链反应（实时PCR）方法进行肺炎链球茵的检测和流行病学调查。方法用实时PCR技术扩增并检测400例临床标本的肺炎链球菌的自溶素（1yt）基因，并将其与传统的培养法进行对比。结果400例，临床标本中，实时PCR检测肺炎链球菌为阳性的有78例（阳性率为18％），传统培养法结果为阳性的有29例（阳性率为7．25％）。结论实时PCR是一种灵敏、特异、快速的检测肺炎链球菌方法，其可用于肺炎链球菌的诊断和流行病学调查。
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| 关 键 词： | 链球菌 肺炎 聚合酶链反应 核酸探针 |
Rapid detection of Streptococcus pneumoniae using TaqMan real-time PCR |
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| Yan Shanhuo,Sun Lei,Fu Kepeng,Long Ju,Ye Haiming,Lao Kegan,Fan Zuqian,Huang Yongxia. Rapid detection of Streptococcus pneumoniae using TaqMan real-time PCR[J]. Foreign Medical Sciences(section of Clinical Biochemistry and Laboratory Medicine, 2014, 0(2): 140-141 |
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| Authors: | Yan Shanhuo Sun Lei Fu Kepeng Long Ju Ye Haiming Lao Kegan Fan Zuqian Huang Yongxia |
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| Affiliation: | (Maternal and Child Health Hospital of Qinzhou ,Qinzhou,Guangxi 535000,China) |
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| Abstract: | Objective To establish real-time PCR method for the identification of S. pneumoniae in clinical and epidemiological studies. Methods 400 clinical specimens were selected. The I.yt gene sequence was amplified and detected by real-time PCR and the results was compared with conventional methods. Results In 400 clinical specimens,78 cases of S. pneumoniae-positive （posi- tive rate was 19 % ） were detected by real-time PCR,while by culture method were 29 cases （positive rate was 7.25 % ）. Conclusion Real-time PCR is a rapid,sensitive and specific assay for the identification of S. pneumoniae. It can be used for the identification and epidemiological investigation. |
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| Keywords: | streptococcus pneumoniae polymerase chain reaction nucleic acid probes |
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