ANGPTL3、GCKR基因单核苷酸多态性与代谢综合征的相关性研究

目的探讨血管生成素样蛋白3（ANGPTL3）、葡萄糖激酶调节蛋白（GCKR）基因单核苷酸多态性（SNP）与代谢综合征（MS）的关系。方法随机选取2012年1月至2013年1月于该校附属医院接受治疗的100例MS患者，选取同期的100例健康体检者作为对照。采用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）分析GCKR基因rs780094，ANGPTL3基因rs112079972个位点的多态性与MS的关系。结果ANGPTL3 rs11207997位点TT基因型携带者高密度脂蛋白（HDL）-C、载脂蛋白（Apo）A1水平明较低；GCKRrs780094 TT基因型携带者三酰甘油（TG）、口服葡萄糖耐量试验（0GTT）3h血糖水平高于CC、CT基因型携带者，0GTT1h胰岛素水平低于CC、CT基因型携带者（P〈0．05）；ANGPTL3 rs11207997位点CT、TT基因型携带者比CC基因型携带者发生MS的风险分别上升l_398和3．180倍；GCKRrs780094 TT基因型携带者发生MS的风险上升2．150倍（P〈0．05）。结论ANGPTL3 rs11207997、GCKRrs780094位点的SNP与MS的发生有密切关系，可以提高MS发病的风险。

The correlation of single nucleotide polymorphisms of ANGPTL3 ,GCKR and metabolic syndrome

Objective To investigate the correlation between single nucleotide polymorphisms （SNP） of ANGPTL3,GCKR and metabolic syndrome （MS）. Methods 100 MS patients and 100 normal subjects in our hospital from January 2012 to January 2013 were selected randomly. PCR-RFLP was used to analyze whether there was a correlation between SNP of GCKR gene rs780094, ANGPTL3 gene SNP rs11207997 and metabolic syndrome. Results The levels of HDL-C and ApoA1 of subjects with ANGPTL3 rs11207997 TT genotype were significantly lower than that of subjects with CC,CT genotype; The triglycerides and OGTT 3 h glucose of people with GCKR rs780094 TT genotype were significantly higher than that of people with the CC,CT genotype,OG- TT l h insulin level was significantly lower than that of people with the CC,CT genotype （P〉0.05）; CT, TT genotypes of AN- GPTL3 rs11207997 increased the risk of developing MS by 1. 398 and 3. 180 times;TT genotype of GCKR rs780094 increased risk occurring MS by 2. 150 times （P〉0.05）. Conclusion The SNPs of ANGPTL3 rs11207997 and GCKR rs 780094 are closely corre- lated to the occurrence of MS and can increase the risk of MS.