犬小肠手术后NO和SOD的变化及意义初探

目的：研究手术创伤愈过程中一氧化氮（nitric oxide,NO)和超氧化物歧化酶（superoxide dismutase,SOD)的变化及其意义,方法：建立犬小肠切除吻合术模型,分伤口愈合正常组和伤口感染组,于术前,术后0,24,48和72h及1wk取静脉血,对NO和SOD的浓度进行测试,结果：手术24h后开始NO的血中浓度逐渐降低,至术后48h为最低水,从术后72h开始逐渐上升至1wk恢复至正常,SOD从术后24h出现明显下降至术后1wk继续下降,感染伤口动物的NO和SOD血中浓度明显低于正常愈合口动物,结论：腹部手术创伤可引起NO和SOD浓度降低,感染伤口则更为显著,提示氧基升高可能对机体的恢复产生损伤作用。

Nitric oxide and superoxide dismutase changes and its implication of following the operation of small bowel of the dogs

AIM To study the changes and the implication of nitric oxide (NO) and superoxide dismutase (SOD) after abdominal operation. METHODS The animal model was established by means of the resection and anastomosis of small bowel, the animals were divided into normal wound and infectious wound groups, the blood samples were collected from the veins before operation and at 0, 24, 48, 72 h and 1wk after operation, and the concentration changes of NO and SOD in serum were observed. RESULTS The concentration of NO decreased at 24 h after operation and reached the lowest level at 48 h, but it increased obviously at 72 h and reached the normal level at 1 wk. The concentration of SOD decreased continuously from 24 h to 1 wk after operation. The concentration of NO and SOD in infectious animals was lower than that in normal wounded animals. CONCLUSION The injury of abdominal operation can decrease the concentration of NO and SOD and it was more obvious in infectious condition which demonstrated that oxygen free radical increasing may play an important role in damage effect on the functional and morphological restore.