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四氯化碳致急性肝损伤后部分肝切除大鼠模型初探
引用本文:冯曜宇,路钢,金辉,韩胜斌,赵凌峰,陈绍春. 四氯化碳致急性肝损伤后部分肝切除大鼠模型初探[J]. 昆明医学院学报, 2009, 30(2): 40-43
作者姓名:冯曜宇  路钢  金辉  韩胜斌  赵凌峰  陈绍春
作者单位:昆明医学院第一附属医院血管外科,云南,昆明,650032
摘    要:
目的建立与临床接近可用于研究病变肝切除后肝再生的急性肝损伤大鼠模型.方法雄性SD大鼠35只.对7只正常大鼠肝脏进行精细解剖,观测其分叶及各叶的比重.其余大鼠平均随机分为4组(每组7只):四氯化碳损伤+35%肝切除组、四氯化碳损伤+65%肝切除组、四氯化碳损伤+80%肝切除组及正常对照组.造模后记录各组术后1d的存活率,存活鼠第3天、第7天经眼眶采血检测肝功(ALT、AST、ALB、TB),麻醉、灌注后取肝组织作组织学观察.结果(1)大鼠肝/体重比为(4.50±0.76)%,可分为7叶,各叶及其占肝的比重分别为:左外叶(36.25±2.96)%;左中叶(9.63±1.30)%;中叶(20.00±1.31)%;右叶(16.13±2.80)%;尾状叶(6.38±1.19)%;前乳头状叶(5.00±0.71)%;后乳头状叶(6.80±2.17)%;(2)依切除范围由低到高各组术后1d的存活率分别为:100%,42.9%,0%;(3)术后3d、7d模型组肝功明显下降,组织学呈急性肝损伤表现.结论四氯化碳急性肝损伤并35%肝切除大鼠模型成功率高,可作为变肝切除后再生的动物模型.

关 键 词:四氯化碳  肝部分切除术  再生  动物模型

A Combined Liver Injury Rat Model:Partial Hepatectomy after Acute Liver Injury Induced by Carbon Tetrachloride
FENG Yao-yu,LU Gang,JIN Hui,HAN Sheng-bin,ZHAO Ling-feng,CHEN Shao-chun. A Combined Liver Injury Rat Model:Partial Hepatectomy after Acute Liver Injury Induced by Carbon Tetrachloride[J]. Journal of Kunming Medical College, 2009, 30(2): 40-43
Authors:FENG Yao-yu  LU Gang  JIN Hui  HAN Sheng-bin  ZHAO Ling-feng  CHEN Shao-chun
Affiliation:FENG Yao - yu, LU Gang, JIN Hui, HAN Sheng - bin, ZHAO Ling - feng, CHEN Shao - chun (Dept. of Vascular Surgery, The 1st Affiliated Hospital of Kunming Medical University, Kunming Yunnan 650032, China)
Abstract:
Objective To establish an animal model which can be used to study the regenerative mechanism of damaged liver which suffered partial hepatectomy. Materials 7 of total 35 male SD rats were used to observe the lobes of liver and to measure the weight of each lobe. The others were randomly divided into 4 groups: CCIA + 35% hepatectomy; CCL4 + 65% hepatectomy; CCL4 + 80% hepatectomy; normal control. The survival rate was recorded 1 day after operation. At the 3rd and 7th day, blood was collected by puncturing orbital vein plexus to determine liver function (ALT/AST/ALB) , and liver tissues were harvested to be examined by histological method. Results (1) Liver of rats was (4.50 ± 0.76)% of body weight. It consisted of 7 lobes, left lateral lobe, left medial lobe, medial lobe, fight lobe, caudate lobe, anterior and posterior discs, which comprised (36.25 ± 2.96)%, (9.63 ± 1.30)%, (20.00 ± 1.31 )%, (16.13 ± 2.80)%, (6.38 ± 1.19)%, (5.00±0.71)%, (6.80 ± 2.17 )% of the liver weight respectively ; (2) The survival rate of 35% , 65%, 80% hepatecotomy was 100% , 42.9% , 0% respectively; (3) Liver function was significantly damaged and liver tissues showed acute pathological changes 3 and 7 days after operation. Conclusions Rat model of padial hepatectomy following acute liver injury induced by CCL4 is a feasible approach to study the regenerative mechanism of damaged liver suffered partial hepatectomy. The appropriate range being excised is about 35%.
Keywords:Carbon tetrachloride  Regeneration  Partial hepatecotomy  Animal model
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