芬太尼后处理联合缺血后适应对心肌缺血再灌注兔超氧化物歧化酶酶蛋白活力浓度和丙二醛浓度的影响

目的：观察芬太尼后处理联合缺血后适应对心肌缺血再灌注兔超氧化物歧化酶（ SOD）酶蛋白活力浓度、丙二醛（ MDA ）浓度的影响。方法32只日本大耳白兔采用随机区组法随机分为四组（每组8只），采用结扎兔左冠状动脉前降支30 min、复灌120 min建立心肌缺血再灌注模型。假手术组：动脉下仅穿线不结扎；缺血再灌注组：直接恢复再灌注；缺血后适应组：缺血后30 min即刻给予3轮复灌30s/缺血30s作为后适应，后恢复再灌注120 min；芬太尼后处理＋缺血后适应组：缺血28 min给予芬太尼5μg/kg后处理后，30 min予以缺血后适应后恢复再灌注。检测各组SOD酶蛋白活力浓度、MDA浓度。结果芬太尼后处理＋缺血后适应组较缺血后适应组外周血MDA（mmol/mL）[（3．52±0．45）比（4．42±0．47）]明显降低（F＝81.619，P＜0．01），SOD酶蛋白活力浓度（U/mL）[（178．38±20．32）比（130．18±20．44）]明显升高（F＝59.359，P＜0.01）。结论芬太尼后处理联合心肌缺血后适应显著降低心肌缺血再灌注兔外周血MDA浓度，提高SOD酶蛋白活力浓度，具有减轻心肌缺血再灌注损伤的作用。

Effects of fentanyl combined with ischemic post-processing on the protein active concentration of SOD and the concentration of MDA in rabbits after myocardial ischemia and reperfusion injury

Objective To explore the effects of fentanyl combined with ischemic post-processing on the pro-tein active concentration of superoside dismutase （ SOD） and the concentration of manlondialdehyde （ MDA） in rab-bits after myocardial ischemia and reperfusion injury.Methods The left anterior descending coronary artery were li-gated for 30 minutes and then reperfusion for 120 minutes to establish myocardial ischemia-reperfusion model of rab-bit.32 Japanese white rabbits were randomly divided into four groups by randomized block method（8 rabbits in each group）：Sham-operated group（group S）：seperate LAD without ligation;ischemia-reperfusion group（group I/R）：re-store reperfusion directly;ischemic post conditioning group（ group IPOC）：restore reperfusion after ischemic post con-ditioning （filling for 30s/ischemia for 30s,three rounds）;combination of fentanyl and ischemic post conditioning group（ group F＋IPOC）：fentanyl post conditioning by ear marginal vein after ischemia 28 minutes,the dose was 5μg/kg, and after 30min,the rabbits was given ischemic post conditioning and then restored reperfusion.Then the differences in the protein active concentration of SOD and in the concentration of MDA were detected.Results The concentra-tion of MDA in the group F＋IPOC were lower than that in the group IPOC[（3.52 ±0.45）mmol/mL vs （4.42 ± 0.47）mmol/mL,F =81.619,P 〈0.01].But the protein active concentration of SOD increased significantly [（178.38 ±20.32）U/mL vs （130.18 ±20.44）U/mL,F=59.359,P〈0.01].Conclusion Fentanyl combined with ischemic post-processing can reduce the concentration of MDA and increase the protein active concentration of SOD significantly,which has the effect to decrease the myocardial ischemia and reperfusion injury.