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| 大鼠局灶性脑缺血再灌注后GDNF mRNA及其蛋白表达的变化 | |
| 引用本文： | 胡跃强,唐农,刘泰,刘尊敬,祝美珍,胡玉英,范立雷. 大鼠局灶性脑缺血再灌注后GDNF mRNA及其蛋白表达的变化[J]. 中国老年学杂志, 2011, 31(20) |
| 作者姓名： | 胡跃强 唐农 刘泰 刘尊敬 祝美珍 胡玉英 范立雷 |
| 作者单位： | 1. 广西中医学院第一附属医院神经内科,广西 南宁,530023 2. 广西中医学院 3. 卫生部中日友好医院神经内科 |
| 基金项目： | 广西自然科学基金，广西中医学院自然科学研究课题 |
| 摘 要： | 目的 观察大鼠脑缺血再灌注后缺血半暗带皮质脑源性神经营养因子(GDNF) mRNA和蛋白的表达变化.方法 采用线栓法制作局灶性脑缺血大鼠(MCAO)模型,用原位杂交法和免疫组化法观察脑缺血后3、6、12 h及l、3、7d共6个时间点GDNFmRNA及其蛋白的表达变化.结果 缺血半暗带GDNFmRNA及其蛋白的表达均于缺血灌注后3h开始明显上升,6h达高峰,12 h表达开始减弱(P＜0.05或P＜0.01),3-d后降至正常水平.结论 脑缺血再灌注后缺血半暗带GDNFmRNA及蛋白表达均明显增加可能参与了脑缺血后神经保护. |
| 关 键 词： | 脑缺血再灌注 GDNF 神经保护 |
Changes of brain-derived neurotrophic factor mRNA and protein expression in the penumbra of rat cortex after focal cerebral ischemia/reperfusion |
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| HU Yue-Qiang,TANG Nong,LIU Tai,et al.. Changes of brain-derived neurotrophic factor mRNA and protein expression in the penumbra of rat cortex after focal cerebral ischemia/reperfusion[J]. Chinese Journal of Gerontology, 2011, 31(20) | |
| Authors: | HU Yue-Qiang TANG Nong LIU Tai et al. |
| Affiliation: | HU Yue-Qiang,TANG Nong,LIU Tai,et al. Department of Neurology,the First Affiliated Hospital of Guangxi University of Traditional Chinese Medicine,Nanning 530023,Guangxi,China |
| Abstract: | Objective To observe the mRNA and protein levels of glial cell line-derived neurotrophic fator(GDNF) in the penumbra of rat after focal cerebral ischemia/reperfusion(I/R).Methods The I/R rat models were established by middle cerebral artery occlusion(MCAO) method to observe the expression variation of GDNF mRNA and protein at 3,6,12 h and 1,4,7 d after I/R by in situ hybridization and immunochemistry method.Results The expressions of GDNF mRNA and protein in the penumbra region were all increased at 3 h aft... |
| Keywords: | Cerebral ischemia/reperfusion GDNF Neuron protection |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |