|
|||||
|
|
| IGF-1对Rh1肉瘤细胞PI3K/Akt/mTOR信号通路的影响 | |
| 引用本文： | 金家红,黄文峰. IGF-1对Rh1肉瘤细胞PI3K/Akt/mTOR信号通路的影响[J]. 中国老年学杂志, 2011, 31(22). DOI: 10.3969/j.issn.1005-9202.2011.22.042 |
| 作者姓名： | 金家红 黄文峰 |
| 作者单位： | 三峡大学医学院,湖北宜昌,443002 |
| 基金项目： | 湖北省卫生厅科研基金指导性项目(JX3C09) |
| 摘 要： | 目的 探讨胰岛素样生长因子(IGF)1对Rh1肉瘤细胞生长活性和PI3 K/Akt/mTOR信号通路的背景变化.方法 常规细胞培养,用无血清培养基消除内源性因子影响27h,再用IGF-1(终浓度为10 ng/ml)刺激72 h,流式细胞仪检测细胞生长活性；另外Western印迹方法观察IGF-1刺激细胞5、10、20、30和60min后Akt(s473)、S6的动态变化.结果 与对照组相比,IGF-1可促进Rh1细胞存活.IGF-1刺激不同时间后S6磷酸化则随着时间的延长逐渐增强；IGF-1亦导致Akt(s473)位点的磷酸化,随时间的延长,磷酸化Akt在5min时达高峰,此后逐渐减弱.结论 Akt、S6等是PI3K/Akt/mTOR信号通路中的重要信号分子,对Rhl细胞而言,在IGF-1刺激下S6有逐渐增强的变化,Akt (s473)位点磷酸化则有减弱的动态变化. |
| 关 键 词： | IGF-1 PI3K/Akt/mTOR |
Effect of IGF-1 on PI3K/Akt/mTOR pathway in Rh1 sarcoma cells |
|
| JIN Jia-Hong,HUANG Wen-Feng. Effect of IGF-1 on PI3K/Akt/mTOR pathway in Rh1 sarcoma cells[J]. Chinese Journal of Gerontology, 2011, 31(22). DOI: 10.3969/j.issn.1005-9202.2011.22.042 | |
| Authors: | JIN Jia-Hong HUANG Wen-Feng |
| Affiliation: | JIN Jia-Hong,HUANG Wen-Feng.Morphology Department,Medical College,China Three Gorges University,Yichang 443002,Hubei,China |
| Abstract: | Objective To investigate the growth activities and the background changes of PI3K/Akt/mTOR pathway with the stimulation of IGF-1 in Rh1 sarcoma cells.Methods Rh1 cells were cultured with 10% FBS RPMI1640 media to firstly abolish the effect of the inner substances,the cells were starved with the pure RPMI1640 for 27 h,then the IGF-1 was added(final concentration 10 ng/ml) for stimulating 5,10,20,30 and 60 minutes separately.The flow cytometer was used to determine the cell growth activities and western blot ... |
| Keywords: | IGF-1 PI3K/Akt/mTOR |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |